SYNERGIC BENEFITS OF ARONIA MELANOCARPA ANTHOCYANIN – RICH EXTRACTS AND ANTIBIOTICS USED FOR URINARY TRACT INFECTIONS
REMO DORNEANU 1, OANA CIOANCĂ 1*, OANA CHIFIRIUC 2, ELENA ALBU 1, CRISTINA TUCHILUŞ 1, CORNELIA MIRCEA 1, IVAN SALAMON 3, MONICA HĂNCIANU 1
1.“Gr. T. Popa” University of Medicine and Pharmacy, 16 Universității Street, Iași, Romania
2.Research Institute of the University of Bucharest, Bucharest, Romania
3.University of Presov, 15 17th November Street, Presov, Slovak Republic
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Anthocyanins are well known for their powerful antioxidative potential, however new insights are discovered every year regarding their health benefits. For the present study, we investigated several anthocyanin-rich extracts in terms of their antimicrobial synergistic effect with commonly used antibiotics in urinary tract infections (UTI). The selected samples, lyophilized powders from Aronia melanocarpa L., were obtained from mature fruits cultivated in ecological environment in the Eastern Carpathians in Romania. The optimized extracts were obtained by selective liquid-liquid extraction in ethanol and acetone and then submitted to analysis. The chemical profile of the polyphenols was established by spectrophotometric and ultra-performance liquid chromatography (UPLC) techniques. To further confirm the value of the extracts we submitted them to some antioxidant tests: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS·+) radical cation scavenging assay and lipoxygenase inhibition test. The minimal inhibitory concentration (MIC) and minimal biofilm eradication concentration (MBEC) of the extracts against several standard and clinical isolated Gram-positive and Gram-negative strains were determined. Our results indicated that the differences induced by the type of solvent used for extraction has a significant impact on the biological activity intensity of each sample.