Romanian Society of Pharmaceutical Sciences
 

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POLYPHENOLIC CONTENT AND ANTIOXIDANT ACTIVITY OF CHRYSANTHEMUM PARTHENIUM EXTRACT

DANIELA HANGANU 1#, DANIELA BENEDEC 1#, LAURIAN VLASE1*, IULIA POPICA1, CONSTANTIN BELE2, OANA RAITA3, ANA-MARIA GHELDIU1, CIPRIAN VALENTIN MIHALI4, VIORICA ȚĂRMURE1

1.“Iuliu Hatieganu” University of Medicine and Pharmacy, 8 V. Babes Street, Cluj-Napoca, Romania
2.University of Agricultural Sciences and Veterinary Medicine, 3-5 Mănăştur Street, Cluj-Napoca, Romania
3.National Institute for Research and Development of Isotopic and Molecular Technologies, 65-103 Donath Street, Cluj-Napoca, Romania
4.Institute of Life Sciences, “Vasile Goldiş” Western University, 86 L. Rebreanu Street, Arad, Romania

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The main objective of this study was the phytochemical and biological analysis of the polyphenolic compounds of Chrysanthemum parthenium. The phenolic compounds were analysed using the reference high performance liquid chromatography (HPLC) method. The evaluation of the polyphenolic content was performed by colorimetric analysis. The ethanolic extract was assessed for the antioxidant activity using three in vitro assay models, the 2,2-diphenyl-1-picrylhydrazylhydrate (DPPH) radical scavenging assay, silver nanoparticles-based assay (SNPs), and an electron paramagnetic resonance (EPR) radicals’ detection. SNPs method was described here for the first time in the C. parthenium extract. Using high performance liquid chromatography - mass spectrometry (HPLC-MS) analysis, phenolic acid derivatives and free aglycones of flavonoids were detected. The extract of C. parthenium with medium total polyphenol content (3.48%) showed a moderate antioxidant activity, as demonstrated by the three methods, and these results could serve as a scientific basis for its therapeutic uses.