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POLYPHENOLIC COMPOUNDS, ANTIOXIDANT ACTIVITY AND NEPHROPROTECTIVE PROPERTIES OF ROMANIAN TARAXACUM OFFICINALE

ALEXANDRA EPURE 1#, ALINA PÂRVU 2#, LAURIAN VLASE 3*, DANIELA BENEDEC 1, DANIELA HANGANU 1, ANA MARIA VLASE 4, ILIOARA ONIGA 1

1Department of Pharmacognosy, Faculty of Pharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, 8 V. Babeș
Street, Cluj-Napoca, Romania 2Department of Phisiopatology, Faculty of Medicine, “Iuliu Hațieganu” University of Medicine and Pharmacy, 8 V. Babeș
Street, Cluj-Napoca, Romania
3Department of Pharmaceutical Technology and Biopharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, 8 V. Babeș Street, Cluj-Napoca, Romania 4Department of Pharmaceutical Botany, Faculty of Pharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, 8 V. Babeș Street, Cluj-Napoca, Romania

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Taraxacum officinale (L.) Weber ex.F.H.Wigg. (dandelion) is a medicinal herb from the Asteraceae family, with a complex chemical composition. The purpose of this research was to determine the polyphenolic profile of Taraxaci herba tincture and to evaluate the antioxidant capacity and the nephroprotective activity. The polyphenolic compounds: total polyphenols content (TPC), total flavonoids content (TFC) and total caffeic acid derivates content (TCAD) were analysed by spectrophotometric and chromatographic methods. Some polyphenols were identified and quantified by HPLC-MS method, with cichoric acid as major compound. The evaluation of the in vitro antioxidant activity was performed using the DPPH• and FRAP methods. The nephroprotective activity of T. herba tincture was evaluated on a rat model with acute kidney injury (AKI) induced by gentamicin. Serum oxidative parameters (total oxidative stress – TOS, oxidative stress index – OSI, total antioxidant capacity – TAC, total nitrites and nitrates – NOx, malondyaldehide – MDA and total thiols – SH), kidney functional parameters (serum creatinine – SCr, serum urea – SU, urinary creatinine – Ucr, urinary urea – UU and creatinine clearance – ClCr) and the transcription factor (Nuclear Factor Kappa B) NF-kB were determined. Although the in vitro test results were modest, in vivo experiments showed a pronounced antioxidant capacity, associated with an important nephroprotective activity