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PHYTOCHEMICAL ANALYSIS OF FUMARIA OFFICINALIS L. (FUMARIACEAE)

RAMONA PĂLTINEAN1, ANCA TOIU2*, JEAN NOËL WAUTERS3, MICHEL FRÉDÉRICH3, MONIQUE TITS3, LUC ANGENOT3, MIRCEA TĂMAŞ1, GIANINA CRIŞAN1

1.Department of Pharmaceutical Botany, Faculty of Pharmacy, "Iuliu Hatieganu" University of Medicine and Pharmacy, 12 Ion Creanga Street, 400010 Cluj-Napoca, Romania
2.Department of Pharmacognosy, Faculty of Pharmacy, "Iuliu Hatieganu" University of Medicine and Pharmacy, 12 Ion Creanga Street, 400010 Cluj-Napoca, Romania
3.Laboratory of Pharmacognosy, Center for Interdisciplinary Research on Medicines (CIRM), Faculty of Medicine, University of Liège, 1, Avenue de l' Hôpital, B-4000- Liege, Belgium

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The present study describes the investigation of active compounds from several samples of Fumaria officinalis L. (Fumariaceae). The identification of the isoquinoline alkaloids (allocryptopine, chelidonine, protopine, bicuculline, sanguinarine, cheleritrine, stylopine and hydrastine) was performed by comparison with reference standards using an HPLC-DAD method, and their quantification by LC-DAD and spectrophotometric methods. The presence of polyphenolic compounds was simultaneously assessed by HPLC. Protopine and sanguinarine were identified in all extracts. The major alkaloids were protopine and chelidonine (258.3 mg/100 g and respectively 94.13 mg/100 g). The spectrophotometric determinations of alkaloids showed minor differences between commercial samples and those harvested from spontaneous flora. The concentration of isoquinoline alkaloids expressed in chelidonine was between 0.69 and 0.76% in all samples. The pattern of phenol carboxylic acids showed the presence of cynarin, chlorogenic, isochlorogenic and ferulic acids. The flavonoids isovitexin, rutin, isoquercitrin and quercitrin were found in all assessed samples of Fumaria officinalis aerial parts.