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ISOLATION, IDENTIFICATION AND REDOX-MODULATION CAPACITY OF HEMOLYMPH’S SUBUNITS FROM RAPANA VENOSA INHABITING THE BULGARIAN BLACK SEA

ALBENA ALEXANDROVA 1*, LUBOMIR PETROV 1, ELINA TSVETANOVA 2, ALMIRA GEORGIEVA 2, LYUDMILA VELKOVA 3, VENTSESLAV ATANASOV 3, ALEKSANDER DOLASHKI 3, PAVLINA DOLASHKA 3, MILKA MILEVA 4

1 National Sports Academy, 21 Acad. St. Mladenov Street, 1700, Sofia, Bulgaria
2 Institute of Neurobiology, Bulgaria Academy of Sciences, 23 Acad. G. Bonchev Street, 1113, Sofia, Bulgaria
3 Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 9 Acad. G. Bonchev Street, 1113, Sofia, Bulgaria
4 “Stephan Angeloff” Institute of Microbiology, Bulgarian Academy of Sciences, 26 Acad. G. Bonchev Street, 1113, Sofia, Bulgaria

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This study aimed to evaluate the radical scavenging potential and metal-chelating activity of fractions from haemolymph with molecular weight (MW) of 1 - 10 kDa, 10 - 50 kDa and 30 - 100 kDa, and hemocyanin subunits RvH1 and RvH2, isolated from Rapana venosa, originating from the Black Sea. The compounds were tested in vitro as inhibitors of superoxide (O2•–) and hydroxyl radicals (•OH) generation, against DPPH radicals, as well as for their iron reduction and chelation capacity and peroxidase activity. We showed a good antioxidant effect of R. venosa haemolymph fractions (especially 30 - 100 kDa) in contrast to hemocyanin subunits. The mechanisms underlying these properties can be assumed to be due at least in part to high proline content, reducing capacity by one-electron transfer, and peroxidase activity.