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IN VITRO BIOCOMPATIBILITY AND IN OVO IRRITANT POTENTIAL SCREENING OF TWO CLEAR ALIGNERS WITH ORTHODONTIC APPLICATIONS

STEFANIA DINU 1,2#, OLTEANU NICOLAE DANIEL 3#, ANAMARIA MATICHESCU 4,5*, ZETU IRINA NICOLETA 6, CAMELIA SZUHANEK 7,8, ANDREEA GEAMANTAN 9,10, ALEXANDRA- DENISA SEMENESCU 9,10, ALINA ANTON 9,10, ROXANA BUZATU 11

1Department of Pedodontics, Faculty of Dental Medicine, “Victor Babeș” University of Medicine and Pharmacy Timisoara, 9 Revoluției 1989 Boulevard, 300041, Timișoara, Romania
2 Pediatric Dentistry Research Centre, Faculty of Dental Medicine, “Victor Babeș” University of Medicine and Pharmacy Timisoara, 9 Revoluției Boulevard, 300041, Timișoara, Romania
3 “Grigore T. Popa” University of Medicine and Pharmacy, 16 Universității Street, 700115, Iași, Romania
4 Department of Preventive, Community Dentistry and Oral Health, Dental Medicine, “Victor Babeș” University of Medicine and Pharmacy Timisoara, 14A Tudor Vladimirescu Avenue, 300173, Timișoara, Romania
5 ⁠Translational and Experimental Clinical Research Centre in Oral Health, “Victor Babeș” University of Medicine and Pharmacy Timisoara, 14A Tudor Vladimirescu Avenue, 300173, Timișoara, Romania
6 Department of Orthodontics, Faculty of Dental Medicine, “Grigore T. Popa” University of Medicine and Pharmacy, 16 Universității Street, 700115, Iași, Romania
7 Department of Orthodontics, Faculty of Dental Medicine, “Victor Babeş” University of Medicine and Pharmacy Timisoara, 9 No., Revolutiei Bv., 300041 Timişoara, Romania
8 Orthodontic Research Centre (ORTHO-CENTER), “Victor Babeș” University of Medicine and Pharmacy Timișoara, Timișoara, Romania
9 Faculty of Pharmacy, “Victor Babeș” University of Medicine and Pharmacy Timișoara, 2 Eftimie Murgu Square, 300041, Timișoara, Romania
10 Research Centre for Pharmaco-Toxicological Evaluations, Faculty of Pharmacy, “Victor Babeș” University of Medicine and Pharmacy Timișoara, 2 Eftimie Murgu Square, 300041, Timișoara, Romania
11 Department of Dental Aesthetics, Faculty of Dental Medicine, “Victor Babeș” University of Medicine and Pharmacy Timișoara, 9 Revoluției Boulevard, 300041, Timișoara, Romania

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Orthodontic conditions represent a serious public health issue, affecting patients of all ages. The current treatment of these conditions implies the utilization of clear aligners (CAs) that improve teeth alignment within the periodontium. The safety and biocompatibility of these dental products used in orthodontics is essential for their use in therapy. Thus, the aim of the present study is to assess the cytocompatibility and safety profile, in vitro (using HaCaT keratinocytes and HGF fibroblasts as experimental models) and in vivo (on the chorioallantoic membrane) of two CAs types (CA1 and CA2) used in orthodontic treatments. The results indicated that the 24 hours exposure to CA1 and CA2 induced no cytotoxicity in HaCaT and HGF cells, the cell viability percentages maintaining over 70%. CA1 and CA2 caused no significant impact on cell morphology, apart from a loss of confluence observed at the dilution of 1:1. Moreover, CA1 and CA2 induced no toxicity at the vasculature of the chorioallantoic membrane, being categorised as non-irritant.