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DETERMINATION OF THE ENANTIOMERIC RATIO OF WARFARIN IN TABLETS BY MULTIVARIATE CALIBRATION AND MODELING OF SPECTROSCOPIC DATA

EDE BODOKI^*, IOANA TIUCA, BOGDAN-CEZAR IACOB, OANA HOSU, RADU OPREAN

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Considering the constant need to develop high performance, selective and robust analytical chiral methods, the aim of the present work was the determination of enantiomeric excess of warfarin by multivariate calibration and modeling of spectral data. Chromatographic and electrophoretic chiral separations have proven their value, offering good selectivity and low limits of quantification; however they may also require lengthy and expensive method development and prolonged analysis times. In this paper we have developed a simple method for the determination of the chiral purity of warfarin from tablets by chemometric spectroscopic calibration, using tbutyl- calix[6]arene as chiral selector. The obtained results were confirmed also by chiral high-performance liquid chromatography.