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COMPOSITION OF SCABIOSA OCHROLEUCA L. EXTRACTS PREPARED BY ULTRASONIC AND MICROWAVE METHODS AND THEIR ANTIRADICAL AND ANTIOXIDANT ACTIVITY ASSESSMENT

ARAILYM MUKANOVA 1*, UBAIDILLA DATKHAYEV 1, RAISSA ABDULLABEKOVA 2, MEREKE ALIMZHANOVA 3, DMITRIY KHRUSTALEV 2, ZHANAR ISKAKOVA 4, GALIYA IBADULLAYEVA 1

1 “Asfendiyarov” Kazakh National Medical University, 88 Tole bi Street, 050000, Almaty, Kazakhstan
2 Medical University of Karaganda, Karaganda city, 40 Gogol Street, 100008, Kazakhstan
3 Center of Physical-Chemical Methods of Research and Analysis, Al-Farabi Kazakh National University, Almaty, Kazakhstan
4 “L.N. Gumilyov” Eurasian National University, 2 Satpaev Street, 010000, Kazakhstan

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Scabiosa ochroleuca L. is one of the promising Kazakh domestic medicinal plants. It has been used in traditional medicine for stomach, ostealgia, fever, tuberculosis, syphilis, eye infections, and as a wound healing agent. Despite its therapeutical applications, there is a lack of its secondary metabolites. The aim of the manuscript is to study the chemical difference between the two extracts prepared by ultrasonic and microwave methods by GC-MS analysis and their antioxidant properties. To determine the possibility of using S. ochroleuca L., we researched the component composition of extracts obtained by ultrasound and microwave methods of the aboveground part of S. ochroleuca L. using the specific GC-MS method, as well as the antiradical and antioxidant activity of the obtained extracts were researched. The GC-MS analysis of the silylized extracts revealed the presence of several major components: catechol, 2-methoxy-4-vinylphenol, 4H-pyran-4-one,2,3-dihydro-3,5- dihydroxy-6-methyl-, and quinic acid. The antiradical activity (DPPH) showed that the ultrasonic extract had a stronger activity (83.9 %). The activity of the ultrasonic and microwave extracts at concentration of 0.75 mg/mL and 1 mg/mL, respectively, was almost the same as the standard butylhydroxyanisole (1 mg/mL), accordingly, using the FRAP method at a concentration of 1 mg/mL, the extracts showed high activity in comparison with the standard sample (ascorbic acid), which proves the prospects of studying this herb.