Romanian Society of Pharmaceutical Sciences

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COMPARATIVE MORPHO-ANATOMICAL STUDIES FOR TWO ASIMINA TRILOBA VARIETIES, PRIMA 1216 AND ITHACA

IRINA TITIRICĂ 1, MARILENA-VIORICA HOVANEȚ 2#, OANA BRINCOVEANU 3*, EMMA ADRIANA OZON 4#, ELIZA OPREA 5#, EUGENIA NAGODĂ 6, LUDOVIC EVERARD BEJENARU 7, LIGIA ELENA DUȚU 8#, ELENA MOROȘAN 9#, CARMMELINA BĂDICEANU 10, FLORENTINA PETCU 1, CORNELIA BEJENARU 11

1 ”Dăbuleni” Research-Development Station for Plant Cultivation on Sandy Soil, 217 Petre Banita Street, 207170, Dolj, Romania
2 Department of Pharmaceutical Botany and Cell Biology “Carol Davila” University of Medicine and Pharmacy, Faculty of Pharmacy, 6 Traian Vuia Street, 020945, Bucharest, Romania
3 National Institute for Research and Development in Microtechnologies, Erou Iancu Nicolae Street, 077190, Voluntari, Ilfov, Romania
4 Department of Pharmaceutical Technology and Biopharmacy “Carol Davila” University of Medicine and Pharmacy, Faculty of Pharmacy, 6 Traian Vuia Street, 020945, Bucharest, Romania
5 Department of Botany and Microbiology, Faculty of Biology, University of Bucharest, 1-3 Aleea Portocalelor Street, 060101, Bucharest, Romania
6 Botanical Garden “D. Brândza”, University of Bucharest, 32 Cotroceni Street, 060114, Bucharest, Romania
7 Department of Pharmacognosy and Phytotherapy, Faculty of Pharmacy, University of Medicine and Pharmacy of Craiova, 2 Petru Rareş Street, 200349, Craiova, Dolj, Romania
8 Department of Pharmacognosy, Phytochemistry and Phytotherapy, Faculty of Pharmacy, “Carol Davila” University of Medicine and Pharmacy, 6 Traian Vuia Street, 020945, Bucharest, Romania
9 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, “Carol Davila” University of Medicine and Pharmacy, 6 Traian Vuia Street, 020945, Bucharest, Romania
10 Department of Clinical Laboratory and Food Safety, Faculty of Pharmacy, “Carol Davila” University of Medicine and Pharmacy, 6 Traian Vuia Street, 020945, Bucharest, Romania
11 Department of Pharmaceutical Botany, Faculty of Pharmacy, University of Medicine and Pharmacy of Craiova, 2 Petru Rareş Street, 200349, Craiova, Dolj, Romania

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Asimina triloba (L.) Dunal (Annonaceae), pawpaw, is known for its edible fruits and therapeutical effects of root, stem, bark, leaves, fruit and seeds (e.g. antimicrobial, antiviral, antitumour, anti-inflammatory). This study compares the morpho-anatomical structure of the stem and leaf of pawpaw, Prima 1216 and Ithaca variety cultivated in Romania. The cross sections (of lamina, vein, petiole and stem) and surface preparation (of lamina) were analysed with the Labophot II Nikon optical microscope and the Nova Nano SEM 630 electronic microscope. Both varieties have similar tissues with some different dimensional parameters of the leaf (length of non-glandular trichomes, area of epidermal cells from adaxial and abaxial epidermis, length and width of stomata, number of stomata/mm2, number of non-glandular trichomes/mm2, number of secretory cells/mm2). The leaf dimensional parameters of the Ithaca variety were larger than Prima 1216 variety, except for the area of epidermal cells from adaxial side of the leaf. Thus, the leaf from the Ithaca variety has more numerous non-glandular trichomes than Prima 1216: 13.17 ± 0.87 pcs/mm2compared to 4.76 ± 0.36 pcs/mm2. Ithaca non-glandular trichomes are longer than Prima 1216 one: 44.31 ± 0.54 µm compared to 34.83 ± 1.04 µm. Statistically significant results (p < 0.05), demonstrate better adaptation of the Ithaca variety at an insufficient water input and high temperature.