Romanian Society of Pharmaceutical Sciences

« Back to Farmacia Journal 4/2022

A NOVEL LC-MS/MS ANALYTICAL METHOD FOR SIMULTANEOUS DETECTION OF ARTICAINE, ARTICAINE ACID, LIDOCAINE AND MONOETHYLGLYCINEXYLIDIDE (MEGX) IN HUMAN PLASMA

TSVETAN TSVETANOV 1*, DESISLAV TOMOV 2

1Department of Oral surgery, Dental Faculty, Medical University of Plovdiv, Bulgaria
2Department of Bioorganic chemistry, Faculty of Pharmacy Medical University of Plovdiv, Bulgaria

Download Full Article PDF

Articaine and lidocaine are the most used local anaesthetic agents in dentistry. There are different methods to identify local anaesthetics and their metabolites in human blood. The liquid-chromatography tandem mass spectrometry is one of them. The purpose of this study was to create an analytical method for the simultaneous detection of articaine, articaine acid, lidocaine and monoethylglycinexylidide (MEGX) in human plasma. The high performance liquid chromatography analysis was performed on a Thermo Dionex Ultimate 3000 LC system (Thermo Fisher Scientific, MA, USA) equipped with a quaternary pump, an autosampler and a thermostat for chromatographic columns. Chromatographic separation was performed under gradient conditions on a core shell Kinetex® 100 x 3.0 mm, 1.7 µm particles analytical column (Phenomenex, CA, USA). The procedure is fast and its appropriate for objective scientific results receiving