Romanian Society of Pharmaceutical Sciences
 

« Back to Farmacia Journal 4/2017

EVALUATION OF POLYQUATERNIUM-1 EFFECTS ON HUMAN CORNEAL EPITHELIAL CELLS

RUI WANG 1, YUJIA ZHAO 2, YANG JIAO 3, XIAOHUA LI 1*

1.Department of Ophthalmology, Hong Qi Hospital of Mu Dan Jiang Medical University, Mu Dan Jiang City, 157011, China
2 .College of Pharmacy, Mu Dan Jiang Medical University, Mu Dan Jiang City, 157011, China
3 .Department of Neurology 1, Hong Qi Hospital of Mu Dan Jiang Medical University, Mu Dan Jiang City, 157011, China

Download Full Article PDF

The aim of this study was to evaluate the antibacterial activity of polyquaternium-1 and its toxicity on human corneal epithelial cells. The antibacterial activity of polyquaternium-1 (PQ-1) samples was measured by nutrient broth dilution method, and the influence of external factors on the stability of PQ-1 was investigated. The cytotoxicity of polyquaternium-1 was evaluated using an in vitro cell model of human corneal epithelium HCEpiC. The PQ-1 samples had a good antibacterial activity, which was not affected by UV irradiation and pH. The antibacterial activity decreased with the increase of temperature and NaCl. In addition, the PQ-1 high concentration had certain toxic effects on HCEpiC, but the cytotoxicity of PQ-1 was low compared with other tested antibacterial agents. Polyquaternium-1 is a good choice as an ophthalmic bacteriostatic agent, being non-irritant and having strong antibacterial activity, low toxicity, and low cytotoxicity on human corneal epithelial cells.