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PHYTOCHEMICAL PROFILING BY LC-HRMS AND ANTIOXIDANT ACTIVITY OF BLUMEA BALSAMIFERA LEAVES EXTRACT BASED ON DIFFERENCES IN THE GROWING LOCATION AND EXTRACTING SOLVENT

RUDI HERYANTO 1,2, ZULAEHA 3, WENI ANINDYA 1, LENY HELIAWATI 3 , DEWI ANGGRAINI SEPTANINGSIH 4, ALFI HUDATUL KAROMAH 1, TRIVADILA 1,2, MIN RAHMINIWATI 2,5 , DYAH ISWANTINI 1,2*, MOHAMAD RAFI 1,2,4

1Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Jalan Tanjung Kampus IPB Dramaga, Bogor 16880, Indonesia
2 Tropical Biopharmaca Research Centre, Institute of Research and Community Services, IPB University, Jalan Taman Kencana No. 3 Kampus IPB Taman Kencana, Bogor 16128, Indonesia
3 Department of Chemistry, Faculty of Mathematics and Natural Sciences, Pakuan University, Bogor 16143, Indonesia
4 Advance Research Laboratory, IPB University, Jalan Palem Kampus IPB Dramaga, Bogor 16680, Indonesia
5 Division of Pharmacology, Department of Anatomy, Physiology and Pharmacology, School of Veterinary Medicine and Biomedical Sciences, IPB University, Kampus IPB Dramaga, Bogor, Indonesia

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This study aims to determine the total phenolic and flavonoid content, antioxidant activity and phytochemical profiling of B. balsamifera leaves using UHPLC-Q-Orbitrap HRMS from two different growth locations and solvent composition extraction. The samples were obtained from Bogor and Sukabumi district, West Java, Indonesia and macerated using water and 70% ethanol. The highest yield (17.52%) was found in 70% ethanol extract of B. balsamifera leaves from Bogor, which also had the highest total phenolic (62.68 mg GAE/g dry powder) and flavonoid (19.06 mg QE/g dry powder) contents. The activities were determined by using DPPH (2,2-diphenyl-1-picrylhydrazyl) (antioxidant activity), CUPRAC (cupric ion reducing anti- oxidant capacity) and FRAP (ferric-reducing antioxidant power). We found the highest antioxidant activity in 70% ethanol extract of B. balsamifera leaves from Bogor with antioxidant capacity values of about 60.35, 1815.25 and 309.91 μmol Trolox/g dry powder. A total of 47 compounds were identified in the B. balsamifera extracts. These compounds were identified as monoterpenes, flavonoids, phenolic acids, coumarin, carboxylic acids, lignans, furans, phthalic acid, sesquiterpene lactone and chalcone.