Romanian Society of Pharmaceutical Sciences
 

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TYROSINE AMMONIA-LYASE ACTIVITY OF SEVERAL AROMATIC PLANTS FROM ALGERIAN SAHARA

ROUKIA HAMMOUDI 1, MOHAMMED LAID TLILI 1,2*, NADIA KHALED 1, DJOUHER KHETTAB 1, MAHFOUD HADJ MAHAMMED 3

1University of Kasdi Merbah Ouargla, Faculty Natural and Life Sciences, Lab. Biogeochemistry of Desert Environments, Algeria
2 University of Hamma Lakhdar El Oued, Faculty Natural and Life Sciences, Algeria
3 University of Kasdi Merbah Ouargla, Faculty Applied Science, Lab. Biogeochemistry of Desert Environments., Algeria

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Tyrosine ammonia-lyase (TAL) is involved in the phenylpropanoids biosynthesis in plants. This study aims to evaluate the activity of TAL extract from several aromatic plants from the Algerian Sahara, namely Petroselinum crispum Mill. (Apiaceae), Mentha piperita L. (Lamiaceae), Medicago sativa L. (Fabaceae) and Deverra scoparia Coss. & Dur. (Apiaceae). The crude extracts were purified by using Mn2+ ions. The optimal pH and temperature were measured by the variation of the reaction medium. The enzymatic activity assay gave variable values; in which the catalytic activity concentration is reduced at each stage of purification. The specific activity is higher in the pure extracts of M. piperita (0.77 ± 0.0839 U/mg) and D. scoparia (0.12 ± 0.0136 U/mg) and in the crude extracts of P. crispum (1.13 ± 0.001 U/mg) and M. sativa (0.68 ± 0.01 U/mg). The latter requires additional purification steps. The enzymatic activity was increased by metal ions and essential oils of the aerial part of Salvia chudaei (Lamiaceae), which are the powerful activators of TAL. This work is the first study on the TAL activity of Algerian plants. These plants are a good source for further studies, leading in future to the elucidation of the structure of bioactive molecules.