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ELICITATION OF ORGANOSULFUR BIOACTIVE COMPOUNDS WITH Fe 3+ AND Zn 2+ IN CELL SUSPENSION CULTURE OF SINGLE GARLIC (ALLIUM SATIVUM L.)

FRIDA KUNTI SETIOWATI 1,3*, WAHYU WIDORETNO 1, SASANGKA PRASETYAWAN 2, BETTY LUKIATI 3

1Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, East Java, Indonesia
2 Chemistry Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, East Java, Indonesia
3 Biology Department, Faculty of Mathematics and Natural Sciences, State University of Malang, Malang, East Java, Indonesia

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Single garlic (Allium sativum L.) is an herbal plant that is rich in organosulfur compounds and widely used for the treatment in health problems such as high blood pressure, hypercholesterolemia, atherosclerosis and cancer. This study aims to investigate the different concentrations effect of Fe3+ and Zn2+ elicitor (0, 0.1, 0.2, 0.3, 0.4, 0.5 mM, respectively) in single garlic cell suspension culture. The formation of suspension culture was done by culturing the callus in Murashige and Skoog liquid medium. The HPLC analysis revealed the presence of 30 organosulfur compounds. The Fe3+ and Zn2+ elicitors can increase the growth of cell suspension culture and production of organosulfur compounds. The highest of fresh weight, dry weight, settled cell volume, growth index were produced in the medium augmented by 0.5 mM Fe3+ and 0.5 mM Zn2+, while organosulfur compounds increase with 0.3 mM Zn2+ (2-fold) and 0.5 mM Fe3+ (1.5-fold). The Fe3+ and Zn2+ metal ions are effective elicitors to increase the production of organosulfur bioactive compounds.