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BIOLOGICAL ACTIVITIES AND PHYTOCHEMICAL SCREENING OF ETHANOL EXTRACTS FROM ADONIS PARYADRICA (RANUNCULACEAE)

OSMAN UCUNCU 1*, CEMALETTIN BALTACI 2, ZEYNEP AKAR 3, AZER OZAD DUZGUN 3, MUSTAFA CUCE 4, ALI KANDEMIR 5

1.Department of Food Processing, Maçka Vocational School, Karadeniz Technical University, 61750 Trabzon, Turkey
2 .Department of Food Engineering, Faculty of Engineering and Natural Sciences, Gümüşhane University, 29100 Gümüşhane, Turkey
3 .Department of Genetic and Bioengineering, Faculty of Engineering and Natural Sciences, Gümüşhane University, 29100 Gümüşhane, Turkey
4 .Department of Food Technology, Şebinkarahisar School of Applied Sciences, Giresun University, 28400 Giresun, Turkey
5 .Department of Biology, Faculty of Arts and Sciences, Erzincan Binali Yıldırım University, 24100 Erzincan, Turkey

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In this study, secondary metabolite profiles of flower, leaf and root of Adonis paryadrica ethanolic extracts were performed by using a newly developed and validated LC-MS/MS method. For this purpose, the LC-MS/MS system was used for the quantitative and qualitative analysis of 37 phytochemicals. The main component of the flower, leaf and root extracts was the quinic acid (160, 111 and 64 mg/kg, respectively). Antioxidant activities were determined using methods such as DPPH (SC50, mg/mL), FRAP (μM TEAC), CUPRAC (μM TEAC), ABTS (SC50, mg/mL) and TPC (GAE, μg/mL). While the highest antioxidant activity value for FRAP, CUPRAC, ABTS and TPC methods was determined in the leaf part of the plant, this value was determined in the flower part for DPPH. The antibacterial effects of the plant extracts were determined by liquid microdilution and agar diffusion methods. As a result, antimicrobial effect of flowers, leaf and all sections of plant extracts were determined except plant root extracts which did not display any activity against bacteria. Antimicrobial activity of the plant flower extract was found to be higher especially against to P. vulgaris with 3.5 mg/mL MIC.