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VIDEO RECORDING AND TIME RESOLVED DIGITAL PARTICLE IMAGE VELOCIMETRY IN EVALUATION OF ANTIAGGREGANT DRUGS

IULIAN SARBU, ERAND MATI, STILIYANA BORISOVA, ALICE PIPEREA-SIANU, IRINA PRASACU^*

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This paper presents an analysis of erythrocytes aggregation and sedimentation processes, using three methods: (i) the sedimentation curve determined by measuring the height of sediment, (ii) the video observation and recording of microscopic images during sedimentation (iii) the analysis of digital particle image velocimetry (PIV) as mathematical method for the estimation of red blood cells aggregates trajectories and velocities. The main finding was the co-existence of ascendant and descendent flow tubes. Single erythrocytes and small aggregates appeared to move up as a counter stream coupled with descending flow of greater aggregates. The mathematical analysis revealed an even more complex picture, including turbulences and formation of local vortexes. Since our experiments with clopidogrel proved an effect of decreasing erythrocytes sedimentation, the PIV method was further applied in analysis of samples containing 4 μg/mL drug. It was observed a significant change in the distribution of speed velocities.