Romanian Society of Pharmaceutical Sciences
 

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THE INFLUENCE OF TWO SYNTHESISED PROSTAMIDES ON OXIDATIVE STRESS INDUCED IN RAW264.7 MACROPHAGE MURINE CELL LINE

SORINA NICOLETA PETRACHE VOICU1, DENISA IOANA UDEANU*2, ANCA DINISCHIOTU1, CORINA ANA IONIȚĂ2

1.Department of Biochemistry, Faculty of Biology, University of Bucharest, 91-95 Splaiul Independenței, sector 5, Bucharest, Romania
2.Department of Clinical Laboratory and Food Safety, Faculty of Pharmacy, University of Medicine and Pharmacy ”Carol Davila”, 6 Traian Vuia Str., sector 2, Bucharest, Romania

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Prostamides are lipid mediators biosynthesised via COX-2 from anandamide (arahidonylethanolamide). The aim of this study was to determine the possible toxicity of prostamides after their peroxidation on RAW264.7 macrophage murine cell line. In the presence of 10μM H2O2 and the studied prostamides, in RAW 264.7 macrophage murine line an oxidative stress occurred, demonstrated by the increase of malondialdehyde (MDA) and protein oxidation levels in a time dependent manner.