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THE INFLUENCE OF MORINGA OLEIFERA OIL ON NEUROBLASTOMA BASAL OXIDATIVE STRESS, INFLAMMATION AND AUTOPHAGY MECHANISM

SOHAIR ALY HASSAN 1, RAMESA SHAFI BHAT 2*, ABEER AL-DBASS 2, ARWA ISHAQ A KHAYYAT 3, AFAF AL ANSARY 4

1 Therapeutic Chemistry Department, Pharmaceutical Industries Research Institute, National Research Centre, Cairo, Egypt
2 Department of Biochemistry, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia
3 Genome Research Chair, Department of Biochemistry, College of Science, King Saud University
4 Autism Centre, Lotus Holistic Alternative Medical Canter, Abu Dhabi

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Despite significant medical advances in neuroblastoma (NB) treatment, therapy resistance is a significant barrier to access to curative cancer treatments. This study investigates the effects of different concentrations of M. oleifera oil on drug resistance and reaction, basal oxidative stress in the SH-SY5Y neuroblastoma cell line, on one hand, and autophagy as a mechanism for tumour and cell survival on the other. First, we profiled the fatty acid composition of seed oils from M. oleifera using gas chromatography–mass spectrometry (GC–MS). Using different concentrations of Moringa olivera oil (50 - 200 μg/mL), the MTT cell proliferation assay was employed to assess cell survival in SH-SY5Y cell lines. Specific antioxidants and proinflammatory markers were estimated; LC3 as a specific marker for autophagy was evaluated, using a flow cytometer. LC3 expression was significantly higher in the SH-SY5Y-treated cell line at the highest concentration (200 μg/mL) compared to the control. A status of dose and time independent reductions in pro-inflammatory cytokines and free radicals as markers of neuroinflammation and oxidative stress were recorded. M. oleifera oil could be used to reduce SH-SY5Y drug resistance initially by antioxidant and anti-inflammatory properties, rather than the essential role of autophagy, which is a novel finding as it shifts focus from autophagy as the central mechanism of action.