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THE EFFECTS OF THE COMBINATION OF ETHANOL BINAHONG LEAF (ANREDERA CORDIFOLIA) AND MOBE LEAF (ARTOCARPUS LAKOOCHA) EXTRACT GEL ON FIBROBLAST AND OSTEOCYTE PROLIFERATION IN WOUND HEALING POST TOOTH EXTRACTION SOCKET ON WISTAR RATS (RATTUS NORVEGICUS)

DENNY SATRIA 1*, NAZLINIWATY 2, OLIVIA AVRIYANTI HANAFIAH 3, RAHMA 3, LUKITA AFRILIA RAHMAH 3, FATHYA AFIFAH RAMADDILA NASUTION 3, MUHAMMAD NUR SASONGKO 3

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Herbal medicines have been widely used throughout the world because of its fewer side effects compared to chemical-based medicines. The purpose of this study was to determine the effect of the combination of ethanol extract gel from binahong leaves and mobe leaves on the proliferation of fibroblasts and osteocytes in socket wounds after tooth extraction in Wistar rats. This in vivo experimental study involved 32 male Wistar rats which were randomly allocated to 8 groups of observations. Tooth extraction was carried out on the lower left incisor. A combined extract gel made of, binahong leaves and mobe leaves in the ratio of 1:1, 1:3, 3:1 was applied to group I to II, III to IV and V to VI respectively, meanwhile, group VII and VIII were given Aloclair® gel and serve as the control group. On the 7th and 21st days after tooth extraction, the rats were sacrified and the proliferation of fibroblasts and osteocytes were observed with Hematoxylin-Eosin staining. Research data were analyzed using one-way ANOVA test. The results showed that the combination group of ethanol extract gel of binahong leaves and mobe leaves with a ratio of 3:1 had the highest number of fibroblast on 7th day (p < 0.05) and the highest number of osteocyte on 21st day compared to the control group (p < 0.05). The experiments showed that combination of ethanol binahong leaves and mobe leaves extract gel had an effect on increasing the proliferation of fibroblasts and osteocytes on the healing of socket wounds after tooth extraction in Wistar rats on the 7th and 21st days.