THE EFFECT OF VITIS VINIFERA L EXTRACT ON EMOTIONAL AND BEHAVIORAL STATUS, OXIDATIVE STRESS AND INFLAMMATION IN A RODENT MODEL OF SURGICAL INDUCED MENOPAUSE
MARIA CRISTINA OLĂNESCU VAIDA VOEVOD, IRINA NECHITA, ALEXANDRA-CRISTINA SEVASTRE-BERGHIAN *, CRISTINA BIDIAN, OANA ALINA HOTEIUC, ALINA MIHAELA TOADER, REMUS MOLDOVAN, NICOLETA DECEA, GABRIELA ADRIANA FILIP
Department of Physiology, “Iuliu Hațieganu” University of Medicine and Pharmacy, 1 Clinicilor Street, 400006, Cluj-Napoca, Romania
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The study aimed to assess the impact of Vitis vinifera L. (VV) extract on behavior, oxidative stress and inflammation in the brain, in an experimental model of menopause induced by ovariectomy (OV). For each subset of experiments (I and II), 20 female Wistar rats were divided into 4 groups (n = 5): animals with sham ovariectomy (SOV) treated with vehicle, SOV treated with VV extract, OV treated with vehicle and OV treated with VV. Vitis vinifera L. extract (30 mg/kg b.w.) or vehicle were orally administered for 21 days (subset I) and 42 days (subset II), after the surgery. OV had a negative impact on memory, behaviour and motor functions of rats, correlated with an increased redox imbalance and minimal changes in the cytokines. 21 days of VV administration triggered mostly non-significant increases of general locomotion and anxiety while after 42 days, VV extract improved general locomotion and diminished significantly the anxiety. MDA levels and tyrosine oxidation decreased after VV treatment in the frontal lobe, both at 21 days and 42 days, while in hippocampus it had controversial effects, with minimal decreases of MDA, high levels of NTZ and variable changes in interleukin levels, especially after 42 days of treatment. Our findings suggest that Vitis vinifera L administration for a long period, may have beneficial effects on both locomotion and emotionality and could also have antioxidant properties in menopause, especially in frontal lobe.