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THE DETERMINATION OF HYPOLIPEMIANT ACTIVITY OF A NEW VEGETAL NATURAL FOOD SUPPLEMENT TO ANIMALS WITH INDUCED HYPERLIPEMIA

GABRIELA DENISA RIZEA^1*, DENISA MIHELE², ŞTEFAN MANEA¹, DANIELA IONESCU¹, VILA RĂDANŢĂ¹

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Scientific studies established the benefic effects of the lipids decrease using dietetically means, synthetic drugs or vegetal extracts. It was proved that the administration of vegetal food oils, containing fatty mono or polyunsaturated acids, decreases the incidence of cardiovascular, hepatic and degenerative diseases. The present work, studied the hypolipemiant activity of a new vegetal, natural supplement having the generic name Hof.Lipomin®. The studies were performed on Wistar rats with Triton WR 1339 induced hyperlipemia. The new supplement, contains the total extracts of garlic (Allium sativum) bulbus, leek (Allium porrum) sheaths and flax-seed (Linum usitatissimum) oil. To determine the hypolipemiant activity we used white adult Wistar-rats, weighting 220 ± 10g, kept in standard conditions in accordance to European demands. Hyperlipemia was induced with Triton WR 1339 at a dose of 10 mg/kg body weight i.p. Twenty hours after the hyperlipemia provocation, a dose of 50mg/200g animal of the food supplement was administrated orally. The determinations were performed simultaneously with oral administration of extracts of garlic and leek extracts in a dose of 50mg/200g animal and flax seed oil in a dose of 1mL/kg b.w. Zocor® was used as a reference substance in concentration of 30 mg/kg b.w. The control group received orally a solution of 0.9% NaCl in a dose of 10mL/kg b.w. animal. After 4 hours the animals were sacrificed under anesthesia with chloroform, the blood was collected and in the separated serum the total serum lipids, cholesterol, LDL cholesterol, HDL cholesterol, and serum triglycerides were determined, using enzymatic and colorimetric methods. The performed studies, emphasized the fact that the food supplement Hof.Lipomin® as well as its components: extracts of garlic, leek and flax seed oil decrease the level of total serum lipids, total cholesterol, LDL cholesterol and serum triglycerides to animals with Triton WR 1339 induced hyperlipemia.