Romanian Society of Pharmaceutical Sciences
 

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THE ADMINISTRATION OF TRICHURIS SUIS OVA EXTRACT DECREASES IL-17/IL-23 LEVELS IN EXPERIMENTAL INFLAMMATORY BOWEL DISEASE

CRISTINA-SORINA CĂTANĂ 1#, CRISTIAN MAGDAȘ 2*, LIVIUŢA BUDIȘAN 3#, ELENA-CRISTINA CRĂCIUN 4#, FLAVIU TĂBĂRAN 5, GEORGIANA DEAK 2, VIRGINIA ANA MAGDAȘ 2, COSMIN PEȘTEAN 6, CIPRIAN OBER 7, IOANA BERINDAN-NEAGOE 3,8, VASILE COZMA 2, ANAMARIA COZMA-PETRUŢ 9, DAN LUCIAN DUMITRAȘCU 10

1Department of Medical Biochemistry, “Iuliu Hatieganu” University of Medicine and Pharmacy, 400349 Cluj-Napoca, Romania
2 Department of Parasitology and Parasitic Diseases, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, Romania
3 Research Centre for Functional Genomics, Biomedicine and Translational Medicine, Institute of Doctoral Studies, “Iuliu Haţieganu” University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania
4 Department of Pharmaceutical Biochemistry and Clinical Laboratory, “Iuliu Haţieganu” University of Medicine and Pharmacy, 400349 Cluj-Napoca, Romania
5 Department of Anatomic Pathology, Necropsy and Forensic Medicine, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, Romania
6 Department of Anesthesiology and Resuscitative Therapy, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, Romania
7 Department of Surgery, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, Romania
8 MEDFUTURE - Research Centre for Advanced Medicine, 400012 Cluj-Napoca, Romania
9 Department of Bromatology, Hygiene, Nutrition, Faculty of Pharmacy, "Iuliu Haţieganu" University of Medicine and Pharmacy, 6 Pasteur, 400349 Cluj-Napoca, Romania
10 2nd Medical Department, “Iuliu Haţieganu” University of Medicine and Pharmacy, 400174 Cluj-Napoca, Romania

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This study investigated the anti-inflammatory effect of Trichuris suis ova extract (TSOE) on four cytokine biomarkers (IL-17, IL-23, IL-6 and IL-10) and on oxidative stress (erythrocyte superoxide dismutase - SOD and whole blood glutathione peroxidase - GPx). The inflammatory bowel disease (IBD) murine model was induced in rats with dextran sulphate sodium (DSS). The experiment was performed on 20 male Wistar rats. Group 1 - control group (CG) received only water; in groups 2 (DSS2), 3 (DSS3) and 4 (DSS4), 3% DSS was administered. The groups 3 and 4 received 100 µg TSOE respectively 200 µg TSOE. The mean level of IL- 23 was significantly higher in the DSS2 group compared to the CG (0.43 pg/mL vs 0.023 pg/mL). After TSOE treatment, the IL-23 concentration was higher in the DSS2 group compared to the DSS3 and DSS4 groups (0.43 vs 0.06 and 0.08 pg/mL, respectively). IL-23 was also paralleled by a decrease in the IL-17 mean level, but without statistical significance (Brown-Forsythe test, F (6; 4.34) = 2.54, p = 0.182). TSOE reduced IL-17/IL-23 levels in the DSS- induced IBD experimental model.