Romanian Society of Pharmaceutical Sciences
 

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SUPERCRITICAL GREEN TECHNOLOGIES FOR OBTAINING GINSENOSIDES FROM FAR-EASTERN WILD GINSENG PANAX GINSENG MEYER USING SFE FOR APPLYING IN DRUG, FOOD AND COSMETIC INDUSTRIES

MAYYA P. RAZGONOVA 1, TATIANA K. KALENIK 2, VALERIY V.VESELOV 3, ALEXANDER M. ZAKHARENKO 1, ALI TAGHIZADEHGHALEHJOUGHI 4, VLAD VUȚĂ 5, FLORICA BĂRBUCEANU 5, BORIS N. IZOTOV 6, ANTONIOS K. STRATIDAKIS 7, ARISTIDIS M. TSATSAKIS 1,3,7*, KIRILL S. GOLOKHVAST 1,8*

1.Far Eastern Federal University, Sukhanova Street, 8, Vladivostok 690950, Russian Federation
2.Biomedical School, Far-Eastern Federal University, Vladivostok, Russia
3.Center of Bioanalytical Investigation and Molecular Design, I.M. Sechenov First Moscow State Medical University, Moscow, Russia
4.Department of Pharmacology, Faculty of Medicine, Ataturk University, 25100 Erzurum, Turkey
5.Institute for Diagnosis and Animal Health, 63 Dr. Staicovici Street, 050557, Bucharest, Romania
6.Department of Analytical Toxicology, Pharmaceutical Chemistry and Pharmacognosy, Sechenov University, 119991 Moscow, Russia
7.Laboratory of Toxicology, School of Medicine, University of Crete, Heraklion 71003, Greece
8.Pacific Geographical Institute FEB RAS, 7 Radio street, Vladivostok, 690014, Russian Federation

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This study focuses on the effect of the modifier, pressure and temperature on the yield of extractable materials on the method of supercritical fluid CO2 extraction of the root of Far-Eastern wild Panax ginseng Meyer. The amounts of biologically active substances (ginsenosides) obtained with the use of small fractions of the modifier are very small, in contrast to large fractions of the modifier used in the experiment. Several experimental conditions were investigated: variations of pressure levels within the range of 200-400 bar, and ethanol percentages from 2 to 5% in the liquid phase at a temperature within the range of 31-70°C.During extraction process with variations in levels of pressure, temperature and amount of modifier, it was found that the presence of the modifier in the extracted system had a significant effect on the amount of extracted ginsenosides. The experimental procedures showed that the main factors that influence the result of ginseng extraction are the density of carbon dioxide, the diffusion coefficient of ginseng oil in carbon dioxide and the volatility of ginseng oil. A direct dependence of the yield of the extract on the amount of modifier was found. High-performance liquid chromatography of all extracts was performed and the quantitative content of ginsenosides in the obtained analytes was established.