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STUDY OF OFLOXACIN – RANDOM BY METHYLATED – β – CYCLODEXTRIN INCLUSION COMPLEX

BLANKA SZÉKELY-SZENTMIKLÓSI^1*, BÉLA TŐKÉS²

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Guest-host interactions of ofloxacin with randomly methylated beta-cyclodextrin (RAMEB) were assessed by complementary analytical techniques. Computer assisted molecular modelling (ChemBio3DUltra 12.0) was utilized for enantiomer-specific characterization of the complex. Ofloxacin - RAMEB complex was prepared by the kneading method and the formation of inclusion complex was confirmed by IR spectroscopy. Differences in their affinity to host molecules resulted in separation of the two enantiomers, thus capillary electrophoresis (CE) proved to be an eligible method for the chiral separation of ofloxacin. The best separation was achieved using a 50 mM phosphate buffer, at pH 3.1, applying a voltage of 20 kV at a temperature of 20°C and 40 mM RAMEB as chiral selector added to the background electrolyte. Under these experimental conditions the chiral separation occurred in 6 minutes.