Romanian Society of Pharmaceutical Sciences
 

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RESEARCHES UPON INDIGENOUS HERBAL PRODUCTS FOR THERAPEUTIC VALORIFICATION IN METABOLIC DISEASES. NOTE II. POLYPHENOLS CONTENT, ANTIOXIDANT ACTIVITY AND CYTOPROTECTIVE EFFECT OF BETULAE FOLIUM DRY EXTRACT

TEODORA COSTEA1, LAURIAN VLASE2*, VIORICA ISTUDOR1, MARIA-LIDIA POPESCU1, CERASELA-ELENA GÎRD1

1.University of Medicine and Pharmacy ,”Carol Davila”, Faculty of Pharmacy, Pharmacognosy, Phytochemistry, Phytotherapy Department, 6 Traian Vuia Street, 020956, Bucharest,
2.University of Medicine and Pharmacy ,”Iuliu Haţieganu“, Faculty of Pharmacy, Department of Pharmaceutical Technology and Biopharmaceutics, 12 I. Creangă Street, 400023, Cluj-Napoca, Cluj

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Among natural compounds, polyphenols are well-known antioxidants with beneficial effects towards endothelial dysfunction, the main cause of vascular complications in type 2 diabetes mellitus (DM 2). In the present study, a Betulae folium dry extract was evaluated for its polyphenols content, antioxidant activity and protective effect against OH• free radicals (generated by H2O2 and FeSO4 reaction) induced oxidative stress in EA.hy926 endothelial human cell line. Spectrophotometric and LC-MS analysis were used for polyphenols determination, 2,2-diphenyl-1-picrylhidrazyl free radical (DPPH) for antioxidant activity and cellular viability (MTT assay) for evaluating the cytoprotective effect. Betulae folium dry extract is a rich source of polyphenols, with antioxidant activity (IC50 = 0.1841mg/mL) and has a modest cytoprotective effect in a narrow concentration range (0.03-0.05 mg/mL).