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QUERCETIN-BIOSAFETY SCREENING FOR FUTURE APPLICATIONS IN THE DENTAL FIELD: AN IN VITRO AND IN OVO INVESTIGATION

HORAȚIU CRISTIAN MANEA 1#, IUSTIN OLARIU 2*, IASMINA-ALEXANDRA PREDESCU 3,4, STEFANIA-IRINA DUMITREL 3,4, ANDREEA KIS 3,4, ALINA HEGHEȘ 5,6#, CAMELIA SZUHANEK 7,8, RAMONA POPOVICI 9, ALINA JOJIC 3, GRATIANA RUSE 3, ROXANA BUZATU 10, STEFANIA DINU 11,12

1Faculty of Nursing, Department I Nursing, University Clinic Clinical Skills, Advanced Research Center in Cardiovascular Pathology and Hemostasology, “Victor Babes” University of Medicine and Pharmacy, 2nd Eftimie Murgu Square, 300041, Timisoara, Romania
“Vasile Goldis” Western University of Arad, Romania, 86 Liviu Rebreanu, 310048, Arad, Romania
3Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy, 2nd Eftimie Murgu Square, 300041, Timisoara, Romania
4Research Centre for Pharmaco-Toxicological Evaluation, Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy, 2nd Eftimie Murgu Square, 300041, Timisoara, Romania
5Department II Pharmaceutical Technology, Faculty of Pharmacy, “Victor Babeș” University of Medicine and Pharmacy, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania
6Formulation and Technology of Drugs Research Center, Faculty of Pharmacy, “Victor Babeș” University of Medicine and Pharmacy, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania
7Department of Orthodontics, Faculty of Dental Medicine, “Victor Babes” University of Medicine and Pharmacy, 9 Revoluției 1989 Boulevard., 300041, Timisoara, Romania
8Orthodontic Research Centre (ORTHO-CENTER), “Victor Babeș” University of Medicine and Pharmacy Timișoara, Timișoara, Romania
9Department of Management, Legislation and Communication in Dentistry, Faculty of Dental Medicine, “Victor Babes” University of Medicine and Pharmacy, 9 Revoluției 1989 Boulevard, 300041, Timisoara, Romania
10Department of Dental Aesthetics, Faculty of Dental Medicine, “Victor Babes” University of Medicine and Pharmacy, 9 Revoluției 1989 Boulevard, 300041, Timisoara, Romania
11Department of Pedodontics, Faculty of Dental Medicine, “Victor Babes” University of Medicine and Pharmacy, 9 Revoluției 1989 Boulevard, 300041, Timisoara, Romania
12Paediatric Dentistry Research Center, Faculty of Dental Medicine, “Victor Babes” University of Medicine and Pharmacy, 9 Revoluției 1989 Boulevard, 300041, Timisoara, Romania

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As conventional treatment causes a series of adverse effects, people tend to opt for safer alternatives for oral health, such as natural oral hygiene products. Quercetin (QUE) is a phytocompound that has garnered attention for its antimicrobial, anti-inflammatory, antioxidant, and anticancer properties, offering several applications in dentistry. Therefore, the present study investigates QUE (20 - 100 μM) in vitro on a human gingival fibroblast cell line (HGF), but also in ovo on the chorioallantoic membrane in terms of irritant potential and impact on blood vessel formation (angiogenesis). The assessments demonstrated that the presence of QUE (20 - 80 μM) does not affect cell viability, and at the highest concentration tested, the viability percentages reach approximately 85%. Complementarily, QUE (20 - 80 μM) did not alter the cell morphology and the appearance of nuclei, whereas the 100 μM dose slightly decreases cell confluency and induces minor dysmorphologies in cell nuclei. Additionally, QUE was classified as a non-irritant (IS = 0.070) and did not alter blood vessel formation. These findings support QUE's biosafety and potential use in dental treatments.