Romanian Society of Pharmaceutical Sciences
 

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PURSLANE (PORTULACA OLERACEA L.) GROWING IN SOUTH KAZAKHSTAN – PHYTOCHEMICAL COMPOSITION OF ULTRASONIC HYDROETHANOLIC EXTRACT AND ITS PHARMACOLOGICAL ACTIVITY

MERUYERT I. TLEUBAYEVA 1, UBAIDILLA M. DATKHAYEV 1, ANNA MALM 2*, ALEKSANDRA JÓZEFCZYK 3, KRZYSZTOF WOJTANOWSKI 3, AIDA M. SEITALIYEVA 4, ARDAK B. JUMAGAZIYEVA 5, ZURIYADDA B. SAKIPOVA 1, KALDANAY K. KOZHANOVA 1, KARLYGHASH T. RAGANINA 1, DIAS N. ZHUMABEK 1

1 School of Pharmacy, S.D. Asfendiyarov Kazakh National Мedical University, Almaty, Kazakhstan.
2 Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Medical University of Lublin, Lublin, Poland.
3 Department of Pharmacognosy with Medicinal Plants Garden, Faculty of Pharmacy, Medical University of Lublin, Lublin, Poland.
4 Department of Fundamental Medicine, Faculty of Medicine and Health, Al-Farabi Kazakh National University, Almaty, Kazakhstan.
5 Microbiology Laboratory, JSC Scientific Center for Anti-Infectious Drugs, Almaty, Kazakhstan

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Aerial parts of Portulaca oleracea L. growing in South Kazakhstan were subjected to ultrasonic extraction with 70% ethanol. The phytochemical composition of the extract was studied using the RP-HPLC/PDA and HPLC-ESI-QTOF-MS methods. The following extract activities were studied in vivo after its oral administration: acute toxicity in outbred white mice, as well as analgesic activity by the formalin test method and anti-inflammatory effect by the carrageenan oedema method in outbred white rats. A total of 19 compounds were identified in the extract by HPLC-ESI-QTOF-MS fingerprinting, mainly organic acids and their derivatives, and phenolic acid derivatives. According to RP-HPLC/PDA data, the main compounds of this extract were derivatives of ferulic and protocatechuic acids (2.502 mg/100 g d. m.). No signs of extract toxicity and mortality were observed, even at a dose of 2000 mg d. m./kg. The extract at doses of 100 mg d. m./kg and 200 mg d. m./kg exerted a significant reduction in the number of pain reactions in both early and late phases, as well as a marked anti-inflammatory effect. These in vivo data demonstrated, for the first time, an analgesic and anti-inflammatory effect following oral administration of ultrasonic hydroethanolic extract from Kazakh species of purslane.