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PROTEIN G MAGNETIC BEADS BASED IMMUNOSENSOR FOR SENSITIVE DETECTION OF ACETAMINOPHEN

OANA HOSU^#, MIHAELA TERTIŞ^#, ROBERT SĂNDULESCU, CECILIA CRISTEA^*

“Iuliu Hațieganu” University of Medicine and Pharmacy, Faculty of Pharmacy, Analytical Chemistry Department, 4 Pasteur Street, Cluj-Napoca, Romania

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An ultrasensitive and specific label-free immunosensor for the detection of acetaminophen is presented. Protein G-magnetic microparticles retained by the external magnetic field at the surface of carbon based screen printed electrodes were used as immobilization platform. The modified beads were then used to capture the anti-acetaminophen antibody immediately followed by the immunological reaction between the antibody and its antigen, acetaminophen. After optimizing all the steps involved in the immunosensor development, a limit of detection of 1.76 μM was obtained on differential pulse voltammetry measurements.