Romanian Society of Pharmaceutical Sciences
 

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POLYPHENOLIC PROFILE AND ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES FROM TWO TRIFOLIUM SPECIES

DANIELA HANGANU1, DANIELA BENEDEC1*, LAURIAN VLASE2, NELI OLAH3, GRIGORE DAMIAN4, RADU SILAGHI-DUMITRESCU4, AUGUSTIN C. MOȚ4, CLAUDIA CRINA TOMA5

1.Department of Pharmacognosy, “Iuliu Hațieganu” University of Medicine and Pharmacy, 12 I. Creangă Street, Cluj-Napoca, Romania
2.Department of Pharmaceutical Botany, “Iuliu Hațieganu” University of Medicine and Pharmacy, 12 I. Creangă Street, Cluj-Napoca, Romania
3.Department of Therapeutical Chemistry, Pharmaceutical Industry and Pharmaceutical Biotechnologies, “Vasile Goldiș” Western University, 86 L. Rebreanu Street, Arad, Romania
4.Department of Chemistry and Chemical Engineering, “Babeş-Bolyai” University, 11 A. Janos Street, Cluj-Napoca, Romania
5.Department of Pharmacognosy, “Vasile Goldiș” Western University, 86 Rebreanu Street, Arad, Romania

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In the present study, flowers and leaves of Trifolium pratense and T. repens were screened for evaluating the polyphenolic contents and the antioxidant and antibacterial activities. A characterization of the main phenolic compounds was carried out using HPLC-MS method. The total polyphenolic and flavonoid content was spectrophotometrically determined. Antioxidant activity was evaluated by using several methods: DPPH (2,2-diphenyl-1-picrylhydrazyl) assay, TEAC (Trolox equivalent antioxidant capacity), HAPX (haemoglobin ascorbate peroxidase activity inhibition) and EPR (electron paramagnetic resonance) method. The polyphenolic profile revealed the presence of common components as p-coumaric acid, ferulic acid, hyperoside, quercetin, luteolin, and other that make the differences (miricetol, quercitrin, isoquercitrin). T. pratense showed better antioxidant and antibacterial activity than T. repens. The quantitative and qualitative differences between the two species of Trifolium could be used as a potential taxonomic marker in order to distinguish the species.