Romanian Society of Pharmaceutical Sciences

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PHYTOCHEMICAL ANALYSIS OF HYPERICUM MACULATUM IN ORDER TO OBTAIN STANDARDIZED EXTRACTS

ILIOARA ONIGA, ANCA TOIU*, DANIELA BENEDEC, IOAN TOMUŢĂ, LAURIAN VLASE

Faculty of Pharmacy, “Iuliu Haţieganu” University of Medicine and Pharmacy, 12, I. Creangă Street, 400010, Cluj-Napoca, Romania

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The aim of this study was to perform a phytochemical analysis of Hypericum maculatum aerial parts harvested from different areas of Transylvania (Romania) and to obtain a dry standardized and concentrated extract which can be easily used to prepare tablets or capsules. Quantitative analyses of total flavonoids and hypericins for five samples of natural product were performed by spectrophotometric methods, whilst the identification and quantitation of polyphenolic compounds by HPLC/MS. The concentrations of flavonoids (3.7 - 5.5%) and total hypericins (0.12 - 0.74%) were determined. Caftaric acid, chlorogenic acid, hyperoside, isoquercitrin, rutin, quercitrin and quercetin were identified and quantified by HPLC/MS. The physicochemical characterization of the dry extract showed a similar content in active compounds as the ethanolic extract from aerial parts.