Romanian Society of Pharmaceutical Sciences
 

« Back to Farmacia Journal 1/2012

NEW MODIFIED ELECTRODES WITH HRP IMMOBILIZED IN POLYMERIC FILMS FOR PARACETAMOL ANALYSIS

ANDREEA CERNAT1*, MIHAELA TERTIS2, SOPHIE GRIVEAU3, FETHI BEDIOUI3, ROBERT SANDULESCU1

1"Iuliu Haţieganu" University of Medicine and Pharmacy, Faculty of Pharmacy, Analytical Chemistry Dept., 4 Pasteur St. 400349, Cluj-Napoca, Romania,
2“Babes-Bolyai” University, Faculty of Chemistry and Chemical Engineering, 11 Arany Janos St. 400028, Cluj-Napoca, Romania
3Unité Pharmacologie Chimique et Génétique et Imagerie, CNRS 8151/INSERM U 1022/Université Paris Descartes/Chimie ParisTech, 11 rue Pierre et Marie Curie, 75231 Paris cedex 05, France

Download Full Article PDF

In order to establish the configurations of biosensors that are able to detect paracetamol in various matrices, it was studied the behaviour of some modified electrodes with an electroconductive polymeric film. Polypyrrole films deposition on glassy carbon and screen-printed electrodes was developped by several methods. The electroanalytical characterization of the deposited films was performed by chronoamperometry in the presence of an enzyme immobilized into the polymeric films. Chronoamperometric study performed with glassy carbon and planar electrodes based on graphite, modified with horseradish peroxidase embedded in polypyrrole films, proved the existence of interactions between the immobilized enzyme and the electroactive species of paracetamol (N-acetyl-p-benzo-quinone imine-NAPQI), enzymatically generated in the presence of hydrogen peroxide.