Romanian Society of Pharmaceutical Sciences
 

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ISOFLAVONOIDS FROM GLYCYRRHIZA SP. AND ONONIS SPINOSA

DANIELA BENEDEC1*, LAURIAN VLASE2, ILIOARA ONIGA1, ANCA TOIU1, MIRCEA TĂMAŞ3, BRÎNDUŞA TIPERCIUC4

1Department of Pharmacognosy, Faculty of Pharmacy, “Iuliu Haţieganu” University of Medicine and Pharmacy, 12 I. Creanga Street, Cluj-Napoca, Romania
2Department of Pharmaceutical Technology and Biopharmaceutics,“Iuliu Haţieganu” University of Medicine and Pharmacy, 12 I. Creanga Street, Cluj-Napoca, Romania
3Department of Pharmaceutical Botany, Faculty of Pharmacy,“Iuliu Haţieganu” University of Medicine and Pharmacy, 12 I. Creanga Street, Cluj-Napoca, Romania
4Department of Pharmaceutical Chemistry, Faculty of Pharmacy,“Iuliu Haţieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania

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In order to identify new sources of isoflavonoids, an analysis was carried out on three species of the Fabaceae family: Glycyrrhiza glabra L., Glycyrrhiza echinata L., Ononis spinosa L., harvested from the Romanian spontaneous flora. The HPLC-MS method was used to investigate the presence of isoflavonoids in the studied plants. In Glycyrrhiza glabra the isoflavonic glycosides like daidzin (0.434x10-3%), genistin (0.672x10-3%), ononin (27.490 x10-3 %), and the aglycon formononetin (16.607 x 10-3%) were found, while in Glycyrrhiza echinata, only formononetin (0.864x10-3%) and ononin (3.904x10-3%) were found. Formononetin was identified in both hydrolyzed solutions. Ononis spinosa, the richest species in isoflavonoids, contains daidzin (0.944x10-3%), genistin (1.173x10-3%), ononin (175.7x10-3%), formononetin (9.499x10-3%) and after hydrolysis, daidzein (0.8196x10-3%), formononetin (113.622x10-3%) and ononin (18.939x10-3%) as residual glycosides.