Romanian Society of Pharmaceutical Sciences
 

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IN VITRO EFFECTS OF SOME BIO-INDOLES ON THE TRANSMEMBRANE POTENTIAL OF JURKAT E 6.1 LIMPHOBLASTS

CRISTINA MANUELA DRĂGOI1, NICULINA MITREA1, ANDREEA LETIŢIA ARSENE1, ALINA CRENGUŢA NICOLAE1, MIHAELA ILIE2

1Department of Biochemistry, Faculty of Pharmacy, “Carol Davila” University of Medicine and Pharmacy, Bucharest
2Department of Toxicology, Faculty of Pharmacy, “Carol Davila” University of Medicine and Pharmacy, Bucharest

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In this paper we proposed to research the biochemical mechanisms of the biocompounds with indolic structure, by investigating their biochemical effects on one very important physico-chemical parameter of the cell membrane, namely the (trans)membrane potential. The aim of the study was the assessment of the membrane depolarizationrepolarization processes induced by some bio-indoles, namely melatonin, serotonin, and tryptophan. To this effect we performed a spectrofluorimetric study using as experimental model the Jurkat E 6.1 lymphoblast cell line and the voltage-sensitive probe bis-(1,3 dibutylbarbituric acid) trimethine oxonol (DiBAC4(3)). The obtained results showed that all the studied bio-indoles have a cell membrane hyperpolarization effect.