Romanian Society of Pharmaceutical Sciences
 

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IN VITRO EFFECTS OF SOME BIO-INDOLES ON THE TRANSMEMBRANE POTENTIAL OF JURKAT E 6.1 LIMPHOBLASTS

CRISTINA MANUELA DRĂGOI1, NICULINA MITREA1, ANDREEA LETIŢIA ARSENE1, ALINA CRENGUŢA NICOLAE1, MIHAELA ILIE2

1Department of Biochemistry, Faculty of Pharmacy, “Carol Davila” University of Medicine and Pharmacy, Bucharest
2 Department of Toxicology, Faculty of Pharmacy, “Carol Davila” University of Medicine and Pharmacy, Bucharest

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In this paper we proposed to research the biochemical mechanisms of the biocompounds with indolic structure, by investigating their biochemical effects on one very important physico-chemical parameter of the cell membrane, namely the (trans)membrane potential. The aim of the study was the assessment of the membrane depolarizationrepolarization processes induced by some bio-indoles, namely melatonin, serotonin, and tryptophan. To this effect we performed a spectrofluorimetric study using as experimental model the Jurkat E 6.1 lymphoblast cell line and the voltage-sensitive probe bis-(1,3 dibutylbarbituric acid) trimethine oxonol (DiBAC4(3)). The obtained results showed that all the studied bio-indoles have a cell membrane hyperpolarization effect.