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IMMUNOCHEMICAL ASSAY OF CHLORAMPHENICOL IN HONEY

IONELA DANIELA MORARIU, LILIANA AVASILCĂI, MĂDĂLINA VIERIU, OANA CIOANCĂ *, MONICA HĂNCIANU

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The veterinary use of chloramphenicol in food-producing animals is forbidden in the European Union. As it is still used for the treatment of infections in animals not bred for consumption, traces of the drug may be found in honey. Developing accessible methods to detect traces of chloramphenicol is of high interest to food residue monitoring and regulation programs. We propose an immunochemical method as an alternative to detect chloramphenicol in honey using Biochip Technology. The sensitivity of the new quantitative determination method for chloramphenicol was high (IC50 = 0.74 μg/kg). The method presented very good accuracy both within the same analytical series and in different analytical series with typical values lower than 15% for concentration levels of 0.5, 1 and 1.5 μg/kg. The decision limit (CCα) of chloramphenicol was 0.37 μg/kg, while the detection capability (CCβ) was 0.42 μg/kg. The method was applied to the determination of chloramphenicol residues in Romanian honey samples.