Romanian Society of Pharmaceutical Sciences

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HISTO-ANATOMICAL RESEARCHES, POLYPHENOLIC PROFILE AND BIOLOGICAL ACTIVITIES OF THE AERIAL PARTS AND TUBERS OF DACTYLORHIZA MACULATA L. SOÓ. (ORCHIDACEAE)

RAMONA PĂLTINEAN 1#, IRINA IELCIU 1#, LAURIAN VLASE 2*, ANDREI MOCAN 1, ANA-MARIA GHELDIU 2, MIHAI BABOTĂ 1, DAN VODNAR 3, GIANINA CRIȘAN 1

1.Department of Pharmaceutical Botany, Faculty of Pharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania
2.Department of Pharmaceutical Technology and Biopharmacy, Faculty of Pharmacy University of Medicine and Pharmacy “Iuliu Hațieganu”, Cluj-Napoca, Romania
3.Department of Food Science, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania

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The present study aimed to provide a histo-anatomical characterization, to evaluate the polyphenolic profile of the tubers and aerial parts belonging to Dactylorhiza maculata L. Soó. and to test their antioxidant capacity and antibacterial effects. Polyphenols were analysed by an HPLC-MS method. The antioxidant activity of the extracts was tested by TEAC and EPR assays. Antibacterial activity was assessed on five bacterial strains. Ethanolic extracts of aerial parts proved to contain mainly isoquercitrin, quercitrin, ferulic and p-coumaric acid, among the tested compounds. Assays of the biological activity revealed a significant antioxidant and antibacterial activity especially for the aerial parts. At the same time, the histo-anatomical features for the main constituents of aerial parts could be established. These results offer a new perspective on D. maculata, proving it as an important source of bioactive compounds.