Romanian Society of Pharmaceutical Sciences
 

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GRAPHENE OXIDE MODIFIED APTASENSOR FOR THE MUCIN 1 DETECTION

BIANCA CIUI1, MIHAELA TERTIŞ1,2, DIANA PEIA1, CECILIA CRISTEA1, ROBERT SĂNDULESCU1*

1.”Iuliu Hațieganu” University of Medicine and Pharmacy, Faculty of Pharmacy, Analytical Chemistry Department, 4 Pasteur Street, Cluj-Napoca, Romania
2.”Ion Chiricuţă” Oncology Institute, 34-36 Republicii Street, Cluj-Napoca, Romania

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An electrochemical aptasensor based on screen printed electrodes modified with graphene oxide was developed for the indirect detection of Mucin 1 which is a tumour biomarker. The signal was obtained after the methylene blue electrochemical reduction. The nanostructured platform was obtained via layer-by-layer deposition of graphene oxide onto the electrode. After the complete optimization and characterization, the platform was incubated with the specific aptamer (5´-GCAGTTGATCTTTGGATACCCTGGTTTTTTTTTTTTTTT-3´) with NH2 terminal group, and then the methylene blue was used as redox probe in order to obtain the electrochemical signal. After the incubation with Mucin1, the aptasensor was successfully tested using electrochemical impedance spectroscopy and differential pulse voltammetry.