Romanian Society of Pharmaceutical Sciences
 

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EVALUATION OF THE BIOCOMPATIBILITY OF LASER IRRADIATED PLANT EXTRACTS USED AS ADJUVANTS IN IRRIGATION AND SANITIZATION OF ROOT CANALS

ANDREI VASILACHE 1#, MARCELA POPA 2#, CRISTINA-CRENGUȚA ALBU 3*, ANCA-OANA DRAGOMIRESCU 1, ADRIANA VASILACHE 1#, MARIA-ANGELICA BENCZE 1, IOANA SUCIU 4, ECATERINA IONESCU 1

1Department of Orthodontics and Dentofacial Orthopaedics, “Carol Davila” University of Medicine and Pharmacy, 37 Dionisie Lupu Street, 020021 Bucharest, Romania
2 Research Institute of the University of Bucharest – ICUB, University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, Romania
3 Department of Genetics, Faculty of Dental Medicine, “Carol Davila” University of Medicine and Pharmacy, 37 Dionisie Lupu Street, 020021 Bucharest, Romania
4 Departments of Endodontics, Faculty of Dental Medicine, "Carol Davila" University of Medicine and Pharmacy, 37
Dionisie Lupu Street, 020021 Bucharest, Romania

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The study aimed to evaluate the biocompatibility of some alcoholic and hydroalcoholic plant extracts, irradiated with laser beams to enhance efficacy, which was used for lavage together with the mechanical treatment of the root canals. In the study, we used seven plant extracts obtained from the Faculty of Biology, University of Bucharest, four preparations of walnut fruit (Juglans regia), two preparations of sage (Salvia Officinalis), and a preparation represented by Echinacea tincture (Echinacea purpurea). The biocompatibility of the seven plant extracts, treated with a 940 nm diode laser beam, in pulsed mode, at powers of 3 W and 5 W, respectively, was evaluated on ATCC (American Type Culture Collection) L929 fibroblasts, by Mosmann’s tetrazolium toxicity (MTT) assay. The analysis of the extracts, from the biocompatibility point of view, highlighted the fact that irradiation influences their characteristics and their ability to react with the metabolic indicators used in the test. There was an improvement in the characteristics of the extracts following laser irradiation, so that although, initially and in some extracts there was a degree of cytotoxicity, after treatment and irradiation with a laser beam with an intensity of 5 W, they become compatible. Due to their antimicrobial properties and reduced cytotoxicity, laser beam irradiated extracts can be used as a root canal irrigator together with, or as an alternative to sodium hypochlorite.