Romanian Society of Pharmaceutical Sciences

« Back to Farmacia Journal 3/2017

EVALUATION OF THE ANTIOXIDANT AND ANTIBACTERIAL POTENTIAL OF BIOACTIVE COMPOUNDS FROM AJUGA REPTANS EXTRACTS

ANCA TOIU1, LAURIAN VLASE2*, ANA MARIA GHELDIU2, DAN VODNAR3, ILIOARA ONIGA1

1.Department of Pharmacognosy, Faculty of Pharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, 8 V. Babeș Street, Cluj-Napoca, Romania
2.Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Pharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, 8, V. Babeș Street, Cluj-Napoca, Romania
3.Department of Food Science, Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, 3-5 Mănăştur Street, Cluj-Napoca, Romania

Download Full Article PDF

The aim of this study was to evaluate the polyphenols and iridoids from Ajuga reptans (Lamiaceae) flower extracts, and their antioxidant and antibacterial activities. Total phenolic content (TPC), total flavonoid content (TFC), total anthocyanin content (TAC) and HPLC/UV/MS were employed for the identification and quantification of polyphenolic compounds. Total iridoid contents (TIC) were determined through a colorimetric method. The bioactive compounds were found in higher quantities in the ethanol extracts. Caffeic, p-coumaric and ferulic acids, quercitrin, luteolin and apigenin were identified by LC/MS in all samples. The antioxidant effects were evaluated by DPPH assay and TPC. A. reptans ethanol extract exhibited a higher antioxidant activity than the methanol extract, and a positive correlation between the antioxidant properties and the concentration of polyphenols was observed. The antimicrobial activity was evaluated by the use of dilution assays; the minimal inhibitory concentration and the minimal bactericidal concentration values were determined. These results indicate that A. reptans flowers contain active compounds with antioxidant and antibacterial potential.