Romanian Society of Pharmaceutical Sciences
 

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EVALUATION OF ANTIPROLIFERATIVE POTENTIAL OF MYRMECODIA PENDANS AND ITS ACTIVITY ON IL-8 SECRETION IN COLON CANCER CELL

AURICA ELISABETA MOATAR 1, CRISTIAN SEBASTIAN VLAD 1*, DALIBORCA CRISTINA VLAD 1, DOINA MIOARA VERDES 2, MARIOARA NICOLETA FILIMON 3, OCTAVIAN BLOJU 3, FLORIN BORCAN 4, CRISTINA ADRIANA DEHELEAN 4, VICTOR DUMITRASCU 1

1.Department of Pharmacology and Biochemistry, Faculty of Medicine, “Victor Babeș” University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041, Timișoara, Romania
2.Department of Morphologic Microscopy, Faculty of Medicine, “Victor Babeș” University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041, Timișoara, Romania
3.Department of Biology and Chemistry, Faculty of Chemistry, Biology and Geography, West University Timișoara, 16 Pestalozzi Street, 300315, Timișoara, Romania
4.Department of Toxicology, Faculty of Pharmacy, “Victor Babeș” University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041, Timișoara, Romania

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Myrmecodya pendans (M. pendans, Rubiaceae family) is a plant with multiple biological properties due to its active compounds. Although it is successfully used in the origin area, in various types of diseases (from infectious disease to diabetes or cancer), just a few studies have analysed its chemical content and its antitumour effects. The present study reports the antiproliferative biological activities of aqueous and ethanolic extracts of M. pendans in colon cancer cells (HCT116) associated with interleukin 8 (IL-8) secretion. Tumour cells were incubated with different concentrations of extract (between 150 and 600 μg/mL) for 24, 48 and 72 h. Cellular metabolic activity was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay. The IL-8 secretion was analysed using the Elisa method. Both extracts exhibited moderate antitumour activity, dose and time dependent in cells incubated with the ethanolic extract 24 and 72 h, with IC50 value of 566.087 μg/mL at 72 hours incubation. The exposure of the cells to aqueous extracts decreased the rate of cell multiplication, dependent on dose and incubation time. The IC50 was 587.95 μg/mL, and 512 μg/mL respectively for a period of 48 and 72 h incubation. The extracts of M. pendans did not influence IL-8 levels in cell cultures.