Romanian Society of Pharmaceutical Sciences

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EVALUATION OF ANTI-MELANOMA, ANTIBACTERIAL AND ANTIOXIDANT EFFECTS OF A PHYTOCHEMICALLY CHARACTERIZED WATER-SOLUBLE FRACTION FROM GREEN COFFEE SEEDS

GRAȚIANA RUSE 1, DIANA-SIMONA TCHIAKPE-ANTAL 1,8, ELENA-ALINA MOACĂ 2,8, ALEXANDRA-DENISA SEMENESCU 2,8, ANA-MARIA VLASE 3, DELIA MUNTEAN 4, FLORINA ARDELEAN 1,8, ALINA-ARABELA JOJIC 5,8, VOICU MIRELA 5,8, ADELINA CHEVEREȘAN 6*, ALINA HEGHEȘ 7

1 Discipline of Pharmaceutical Botany, Faculty of Pharmacy, “Victor Babeș” University of Medicine and Pharmacy Timișoara, 2 Eftimie Murgu Square, 300041, Timișoara, Romania
2 Discipline of Toxicology, Drug industry, Management and Legislation, Faculty of Pharmacy, “Victor Babeș” University of Medicine and Pharmacy Timișoara, 2 Eftimie Murgu Square, 300041, Timișoara, Romania
3 Discipline of Pharmaceutical Botany, Faculty of Pharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, 8 Victor Babeș Street, Cluj-Napoca, Romania
4 Discipline of Microbiology, Faculty of Medicine, “Victor Babeș” University of Medicine and Pharmacy Timișoara, 2 Eftimie Murgu Square, 300041, Timișoara, Romania
5 Discipline of Pharmacology-Pharmacotherapy, Faculty of Pharmacy, “Victor Babeș” University of Medicine and Pharmacy Timișoara, 2 Eftimie Murgu Square, 300041, Timișoara, Romania
6 Discipline of Pharmacology, Faculty of Medicine, “Victor Babeș” University of Medicine and Pharmacy Timișoara, 2 Eftimie Murgu Square, 300041, Timișoara, Romania
7 Discipline of Pharmaceutical Technology, Faculty of Pharmacy, “Victor Babeș” University of Medicine and Pharmacy Timișoara, 2 Eftimie Murgu Square, 300041, Timișoara, Romania
8 Research Centre for Pharmaco-Toxicological Evaluations, Faculty of Pharmacy, “Victor Babeș” University of Medicine and Pharmacy Timișoara, 2 Eftimie Murgu Square, 300041, Timișoara, Romania

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The green seeds of Coffea arabica are appreciated for the promotion of health and wellness. The current research focuses on the phytochemicals and bioactivity of the water-soluble fraction prepared from green coffee seeds. The first steps were to characterize the extract with the aid of HPLC, to record the FT-IR spectrum and to evaluate the content in total polyphenols. Subsequently, the antioxidant and antibacterial effects were determined. The last part of the research concerns the effect of the extract on the melanoma cell line A375. The water-soluble fraction, rich in chlorogenic acid, displayed a modest antibacterial effect, stronger against the fungus Candida parapsilosis than against the tested bacteria. It reduced the viability of A375 cells in a concentration-dependent manner, decreased cell number and confluence, presented a negative impact on the cell membrane integrity, and induced apoptosis. These effects warrant further investigations into the potential of the water-soluble fraction in the nutraceutical and dermatologic fields.