Romanian Society of Pharmaceutical Sciences
 

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EFFECTS OF ZOLEDRONIC ACID ON OSTEOBLAST PROLIFERATION AND IGF-1 EXPRESSIONS IN RATS IN VITRO

JIANMING HUANG 1, QIANG ZHAO 2, KUIJIE WANG 3, CHEN CHEN 4, SULI XIE 5, DEDING LIU 5*

1.Department of Orthopedics, The Affiliated Chenggong Hospital of Xiamen University (The 174 Hospital of PLA), 361003, People's Republic of China
2.Department of Orthopedics, Zhangqiu of Jinan City People's Hospital, 250200, People's Republic of China
3.Department of Internal Neurology, Zhangqiu of Jinan City People's Hospital, 250200, People's Republic of China
4.Department of Orthopedics Trauma Surgery, General Hospital of Jinan Military Command, 250031, People's Republic of China
5.Department of Joint Orthopedics, 153 Central Hospital of the PLA, Zhengzhou City, 450000, People's Republic of China

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This study aimed to investigate the effects of different concentrations of zoledronic acid (ZA) on the proliferation of rat osteoblasts and the expression of insulin-like growth factor-1 (IGF-1) in vitro, and to provide a theoretical basis for its clinical use. Rat osteoblasts were divided in 5 groups: 1 control group cultured in normal culture medium and 4 experimental groups cultured in normal culture media with 10-4 mol/L, 10-5 mol/L, 10-6 mol/L, 10-7 mol/L ZA for 7 days. The proliferation of the primary cultured rat osteoblasts was evaluated by MTT (methyl thiazolyl tetrazolium) assay. The expression of IGF-1 was evaluated by ELISA method, and the ALP (alkaline phosphatase) was determined to detect the viability of basic phosphatase. ZA in concentrations above 10-5 mol/L inhibits the osteoblast proliferation. When the concentration of ZA was between 10-6 and 10-7 mol/L, no effect was observed on the cell proliferation. ZA inhibits osteoblasts ALP production at concentrations above10-6 mol/L. At concentrations between 10-6 and 10-7mol/L ZA have no effect on the expression of IGF-1. ZA is closely related to proliferation and IGF-1 expressions of rat osteoblasts.