Romanian Society of Pharmaceutical Sciences
 

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EFFECTS OF ZOLEDRONIC ACID ON OSTEOBLAST PROLIFERATION AND IGF-1 EXPRESSIONS IN RATS IN VITRO

JIANMING HUANG 1, QIANG ZHAO 2, KUIJIE WANG 3, CHEN CHEN 4, SULI XIE 5, DEDING LIU 5*

1.Department of Orthopedics, The Affiliated Chenggong Hospital of Xiamen University (The 174 Hospital of PLA), 361003, People's Republic of China
2 .Department of Orthopedics, Zhangqiu of Jinan City People's Hospital, 250200, People's Republic of China
3 .Department of Internal Neurology, Zhangqiu of Jinan City People's Hospital, 250200, People's Republic of China
4 .Department of Orthopedics Trauma Surgery, General Hospital of Jinan Military Command, 250031, People's Republic of China
5 .Department of Joint Orthopedics, 153 Central Hospital of the PLA, Zhengzhou City, 450000, People's Republic of China

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This study aimed to investigate the effects of different concentrations of zoledronic acid (ZA) on the proliferation of rat osteoblasts and the expression of insulin-like growth factor-1 (IGF-1) in vitro, and to provide a theoretical basis for its clinical use. Rat osteoblasts were divided in 5 groups: 1 control group cultured in normal culture medium and 4 experimental groups cultured in normal culture media with 10-4 mol/L, 10-5 mol/L, 10-6 mol/L, 10-7 mol/L ZA for 7 days. The proliferation of the primary cultured rat osteoblasts was evaluated by MTT (methyl thiazolyl tetrazolium) assay. The expression of IGF-1 was evaluated by ELISA method, and the ALP (alkaline phosphatase) was determined to detect the viability of basic phosphatase. ZA in concentrations above 10-5 mol/L inhibits the osteoblast proliferation. When the concentration of ZA was between 10-6 and 10-7 mol/L, no effect was observed on the cell proliferation. ZA inhibits osteoblasts ALP production at concentrations above10-6 mol/L. At concentrations between 10-6 and 10-7mol/L ZA have no effect on the expression of IGF-1. ZA is closely related to proliferation and IGF-1 expressions of rat osteoblasts.