Romanian Society of Pharmaceutical Sciences
 

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EFFECTS OF URINASTATIN ON T CELL IMMUNOLOGICAL FUNCTION IN A MURINE MODEL OF SEPSIS

QIUQI GAO 1,2, JINGYE PAN 2, WEIWEI WANG 2, XIANGYANG LIN 3, HUIYA HUANG 2, YAJING PAN 4, LEI SU 5*

1.The First School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong 510010, China
2 .Department of Intensive Care Unit, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, China
3 .Department of Clinical laboratory, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, China
4 .Department of Intensive Care Unit, Wenzhou people’s Hospital, Wenzhou, Zhejiang 325000, China
5 .Department of Intensive Care Unit, Affiliated General Hospital of Southern Theatre Command of PLA, Southern Medical University, Guangzhou, Guangdong 510010, China

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This study aimed to observe the effects of urinastatin (UTI) on the immunological function on a murine model of sepsis. Ninety-six adult CD-SD (Sprague-Dawley) rats were divided into three groups: sham operation group (Sham group), CLP model group (CLP group), and UTI treatment group (UTI group). The murine model of sepsis was prepared by caecal ligation and puncture (CLP) and the survival rate of rats in each group was observed and recorded. The intestinal flora in the faces and the levels of tumour necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-17 (IL-17), plasma endotoxin and the T cell subsets were determined at 6 h, 12 h, 24 h and 48 h after the modelling. The results showed that the levels of TNF-α, IL-6, IL-17, and plasma endotoxin in the CLP group increased significantly compared with the Sham group, indicating that sepsis caused an inflammatory response in the body. After UTI treatment, the levels of TNF-α, IL-6, IL-17, and plasma endotoxin began to decrease, indicating that UTI treatment of sepsis can reduce the highly inflammatory response of the body. Moreover, the overall mortality rate of rats in the UTI group was significantly lower than that of the CLP group. Immune suppression did not occur immediately after CLP in the UTI group, and the CD4+ and CD4+/CD8+ increased with the prolongation of the medication time. In conclusion, UTI can alleviate the excessive inflammatory response, reduce the inflammatory damage, increase the number of CD4+ T cells, and improve the state of immune disorder through its anti-inflammatory effect.