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EFFECT OF PLANT GROWTH REGULATORS ON THE PRODUCTION OF FLAVONOIDS BY CELL SUSPENSION CULTURES OF DIGITALIS LANATA

CRISTINA BOTA^1*, CONSTANTIN DELIU²

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In this paper we investigated the effect of some phytohormones on the production of flavonoids in cell suspension cultures of Digitalis lanata. There were used two cell lines and different combinations and concentrations of phytohormones. The highest flavonoids amounts were obtained after 14 days of treatment. Among the tested combinations, the highest yield of flavonoids, 7.58 mg/g d.w. (dry weight) was obtained, for line 13C-100, with 0.1 mg/L IAA (β-indoleacetic acid) + 1 mg/L BA (6-benzylaminopurine). For line 11, the most significant increase in flavonoid amount was achieved with 0.1 mg/L NAA (α-naphtaleneacetic acid) + 1 mg/L BA.