Romanian Society of Pharmaceutical Sciences

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DYNAMIC ACTIVITY OF SOME ANTIOXIDANT ENZYMES IN PRIMARY CULTURES OF NEURONS UNDER HYPOXIA AND ISCHEMIA

LIVIA GRATIELA MIHAI1*, NICULINA MITREA2, RALUCA IOANA PAPACOCEA1, ANCA IOANA BADARAU1

1University of Medicine and Pharmacy “Carol Davila “, Faculty of Medicine, Physiology I Department, Bucharest, Romania.
2University of Medicine and Pharmacy “Carol Davila”, Faculty of Pharmacy, Biochemistry Department, Bucharest, Romania.

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Models of oxidative stress are important in elucidating pathologies associated with increased levels of reactive oxygen species, including stroke and neurodegenerative diseases, such as Alzheimer and Parkinson diseases. Cerebellar granule neurons primary cultures became the most popular model for the in vitro studies, bringing information about the development functionlity and pathology. In this paper, we present the results of the forme antioxidant enzymes activities (catalase, superoxide dismutase, glutathione peroxidase) under oxidative stress conditions induced by hypoxia and ischemia. The spectrophotometric methods used, allow the quantification of the production of reactive oxygen species after hypoxic incubation at 37ºC and ischemia, within one hour, two hours, and three hours by monitoring the activity of antioxidant enzymes.