Romanian Society of Pharmaceutical Sciences

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DEVELOPMENT AND VALIDATION OF A NEW METHOD BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY FOR THE QUANTITATIVE ANALYSIS OF MAGNOLOL LOADED IN SILICA PARTICULATE SYSTEMS

ALINA ȘTEFANACHE1, LĂCRĂMIOARA OCHIUZ1*, MARIA IGNAT2, ANDREEA CREȚEANU1, GLADIOLA ȚÂNTARU1

1.”Grigore T. Popa” University of Medicine and Pharmacy Iași, 16 Universității Street, 700115, Iași, Romania
2.“Al. I. Cuza" University, Faculty of Chemistry, 11 Carol I Bvd., 700506, Iași, Romania

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In order to analyse magnolol (MG) in modern pharmaceutical forms, we developed and validated a new high performance liquid chromatography (HPLC) method with UV detection. We optimized the working conditions for the chromatographic system and recorded the chromatograms at the wavelength of 290 nm. The method was validated by the determination of the following parameters: method repeatability and intermediate precision for the concentration of 0.5 mg/mL for which we obtained RSD values of ≤ 2.5 for peak area, retention time and concentration, method linearity in the concentration range 0.025 - 1.5 mg/mL; calibration curve equation for MG (y = 492.05x + 6.5518) (R2 = 0.9985); detection limit of 0.00988 mg/mL and limit of quantification of 0.02993 mg / mL; method robustness for MG concentration of 0.5 to 1 mg/mL; accuracy of method, for which the achieved recovery rate was in the range 98.42 - 103.83%. The method was tested by the determination of MG loaded in nanoporous silica. Results showed a 96.57% degree of retrieval and confirm the hypothesis that this method can be used for the quantitative determination of MG loaded in silicate matrices as modified release formulations.