Romanian Society of Pharmaceutical Sciences

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DEVELOPMENT AND CHARACTERISATION OF IMPLANTABLE SANDWICH STRUCTURES MADE OF LITHIUM-DOPED BIOLOGICAL-DERIVED HYDROXYAPATITE AND CIPROFLOXACIN

OANA ANDREEA ZUREIGAT 1, JOHNY NEAMȚU 1, LIVIU DUTA 2*, MĂDĂLINA ICRIVERZI 3, PAULA FLORIAN 3, LIVIA-ELENA SIMA 3, GABRIELA DORCIOMAN 2, VALENTINA GRUMEZESCU 2, ROXANA-MARIA BĂLĂȘOIU 1, SANDRA ALICE BUTEICĂ 1, RENATA MARIA VĂRUȚ 1, OANA ELENA NICOLAESCU 1

1 University of Medicine and Pharmacy, Faculty of Pharmacy, 2 Petru Rareş Str., 200349 Craiova, Dolj County, Romania
2 National Institute for Laser, Plasma and Radiation Physics, 409 Atomiștilor Str., 077125 Măgurele, Romania
3 Institute of Biochemistry of the Romanian Academy, 296 Spl. Independenței, 060031 Bucharest, Romania

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To address the limitations of synthetic hydroxyapatite (HA), which lacks the precise chemical composition of natural bone minerals, alternative, cost-effective production methods from sustainable biological resources, termed BioHA, have been investigated. Incorporating CIP into BioHA-based implant coatings enables localised, high-concentration antibiotic release directly at infection sites, thereby reducing systemic exposure and associated side effects. In this study, we utilised Matrix-Assisted Pulsed Laser Evaporation (MAPLE) for the deposition of CIP, achieving sustained concentrations above the minimum inhibitory concentration for Staphylococcus aureus throughout a 21-day release period. Biocompatibility assays revealed that hMSCs adhered to titanium (Ti) surfaces with CIP-loaded coatings exhibited significantly reduced proliferation rates compared to control Ti surfaces. Moreover, indirect cytotoxicity assessments indicated that, while no significant cytotoxic effects were induced on any of the surfaces, a modest reduction in hMSC proliferation was observed in the presence of CIP. These findings highlight the complex interplay between antimicrobial agents and stem cell behaviour, underscoring the necessity for careful consideration in designing biomaterials for clinical applications.