Romanian Society of Pharmaceutical Sciences
 

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CYTOTOXIC EFFECT OF CHLOROFORM EXTRACTS FROM TANACETUM VULGARE, T. MACROPHYLLUM AND T. CORYMBOSUM ON HELA, A375 AND V79 CELL LINES

BIANCA IVĂNESCU 1#, CARMEN ELENA POP 2*, LAURIAN VLASE 3#, ANDREIA CORCIOVĂ 4, DANIELA GHERGHEL 5, GABRIELA VOCHITA 5, CRISTINA TUCHILUȘ 6, CONSTANTIN MARDARI 7, COSMIN MIHAI TEODOR 8

1.Department of Pharmaceutical Botany, “Gr. T. Popa” University of Medicine and Pharmacy, 16 Universității Street, Iași,
Romania
2 .Department of Drug Industry and Pharmaceutical Biotechnologies, “Iuliu Hațieganu” University of Medicine and
Pharmacy, 12 Ion Creangă Street, Cluj-Napoca, Romania
3 .Department of Pharmaceutical Technology and Biopharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, 12 Ion Creangă Street, Cluj-Napoca, Romania
4 .Department of Drugs Analysis, “Gr. T. Popa” University of Medicine and Pharmacy, 16 Universității Street, Iași, Romania
5 .Institute of Biological Research, 47 Lascăr Catargi Street, 700107, Iași, Romania
6 .Department of Microbiology, “Gr. T. Popa” University of Medicine and Pharmacy, 16 Universității Street, Iași, Romania
7 .“Anastasie Fătu” Botanical Garden, “Alexandru Ioan Cuza” University of Iași, 7-9 Dumbrava Roșie Street, Iași, Romania
8 .Advanced Centre for Research and Development in Experimental Medicine, “Gr. T. Popa” University of Medicine and Pharmacy, 9 Kogălniceanu Street, Iași, Romania

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The effect of Tanacetum extracts on cell viability was assessed by MTT method on HeLa (human cervical epithelioid carcinoma), A375 (human malignant melanoma) and V79 (Chinese hamster pulmonary fibroblasts). Apoptosis, cell cycle analysis, and genotoxicity tests were performed to identify the possible mechanism of action. Also, the antimicrobial activity was investigated, and LC-MS analysis of extracts was carried out. Tanacetum extracts substantially reduced the viability of all tested cells, normal cells being more sensitive than cancer cells. T. vulgare and T. macrophyllum extracts induced apoptosis in normal cells V79, while none of the extracts induced apoptosis on HeLa or A375 neoplastic cells. T. vulgare extract arrested cell cycle progression of V79 and A375 cells at the G2/M phase and exhibited mild genotoxicity on melanoma cells A375. All extracts showed moderate antimicrobial activity against tested Gram-positive bacteria and fungi.