Romanian Society of Pharmaceutical Sciences
 

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CYTO-HISTOPATHOLOGICAL AND PCR CORRELATION OF CATTLE PULMONARY HYDATID DISEASE

FLORICA BĂRBUCEANU 1,2*, GABRIEL PREDOI 2, CORINA-BEATRIS ANCUCEANU 1, STELIAN BĂRĂITĂREANU 2, ȘTEFANIA-FELICIA BĂRBUCEANU 3

1.Institute for Diagnosis and Animal Health, 63 Dr Staicovici D. Nicolae Street, 050557, Bucharest, Romania
2 .University of Agricultural Science and Veterinary Medicine, Faculty of Veterinary Medicine, 105 Splaiul Independenței, 050097, Bucharest, Romania
3 .Department of Organic Chemistry, “Carol Davila” University of Medicine and Pharmacy, Faculty of Pharmacy, 6 Traian Vuia Street, 020956, Bucharest, Romania

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Hydatid disease is a severe zoonosis caused by metacestodes (the larval stage of a tapeworm) of the genus Echinococcus. Considering the complex ultrastructure of the cyst, the cyto-histopathological analysis of the hydatid cyst layers requires the use of various histochemical staining techniques. This paper is a cyto-histopathological and PCR analysis of the uninfected and infected pulmonary hydatid cyst membrane structures, conducted between 2009 and 2019 on a total of 2123 lung samples of cattle 2-18 years old. The open infected hydatid cyst, identified in lungs from cattle, can be considered, from the cyto-histopathological point of view, as a diffuse granulomatous hydatid proliferation. PCR was a reliable and valuable tool in the E. granulosus rapid diagnosis.