Romanian Society of Pharmaceutical Sciences

« Back to Farmacia Journal 1/2019

COMPARATIVE PHYTOCHEMICAL AND ANTIOXIDATIVE CHARACTERIZATION OF TRIFOLIUM PRATENSE L. AND OCIMUM BASILICUM L.

INA-ANDREEA ANTONESCU (MINTAS) 1, TUNDE JURCA 2, FELICIA GLIGOR 3, IZABELA CRACIUN 3, LUMINITA FRITEA 1, EVA BRIGITTA PATAY 4, MARIANA MURESAN 1, DENISA IOANA UDEANU 5, CORINA ANA IONIȚĂ 5, ANGELA ANTONESCU 1*, FLORIAN BODOG 6

1.University of Oradea, Faculty of Medicine and Pharmacy, Department of Preclinical Disciplines, Oradea, Romania
2.University of Oradea, Faculty of Medicine and Pharmacy, Department of Pharmacy, Oradea, Romania
3.University’’Lucian Blaga’’, Sibiu, Faculty of Medicine and Pharmacy, Department of Pharmacy, Sibiu, Romania
4.University of Pecs, Faculty of Pharmacy, Department of Pharmacognosy, Pecs, Hungary
5.University of Medicine and Pharmacy “Carol Davila”, Faculty of Pharmacy, Department of Clinical Laboratory and Food Safety, Bucharest, Romania
6.University of Oradea, Faculty of Medicine and Pharmacy, Department of Surgery, Oradea, Romania

Download Full Article PDF

The phytochemical composition of Trifolium pratense L. and Ocimum basilicum L. was investigated by using chromatographic and spectrophotometric methods. T. pratense extract was rich in ferulic acid (237.48mg/100g dry weight) and chlorogenic acid (158.50 mg/100g dry weight), meanwhile O. basilicum extract presented the highest concentration in ferulic acid (437.58 mg/100g dry weight). The determination of total phenolic and flavonoid contents indicated that O. basilicum contained the highest amounts (67.89mg GAE/100g dry extract and 6.49 mg QE/mL extract respectively), aspect which was corelated with its strongest antioxidant activity assessed by DPPH (2,2-diphenyl-1-picrylhydrazyl), FRAP (ferric reducing antioxidant power) and CUPRAC (cupric reducing antioxidant capacity) methods.