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COMBINATION OF SIX COMPOUNDS SYNERGISTICALLY BOOSTS ANTIOXIDANT EFFECTS EX VIVO

JELENA KOTUR-STEVULJEVIĆ 1, JELENA S. SAVIĆ 2*, TAMARA ANTONIĆ 1, AZRA GUZONJIĆ 1, MILENA R. SIMIĆ 3, TAMARA GOJKOVIĆ 1, NATAŠA BOGAVAC-STANOJEVIĆ 1

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In this ex vivo study, we aimed to evaluate the antioxidant properties of the N-acetylcysteine, zinc, manganese, selenium, vitamin C and vitamin D in human serum, as well as the effect of their mixture present in the product BiVits® ACTIVA Recovery (Abela Pharm d.o.o. Belgrade, Serbia). The serum pool was formed by collecting samples from individuals with healthy profiles. To assess the redox status, total oxidant status (TOS), prooxidant-antioxidant balance (PAB), total antioxidant status (TAS), superoxide dismutase (SOD) activity and total sulfhydryl groups (SHG) concentration were determined. Subsequently, antioxidant score (AS), prooxidant score (PS), and general oxy score (OS), were calculated. These scores served as valuable indicators of the overall redox balance. The results of this study showed that although all components exhibited antioxidant properties individually, the mixture of these components contained in the product BiVits® ACTIVA Recovery had a much more pronounced antioxidant potential both after short- and long-term incubation in conditions that mimic physiological environment and in an oxidative stress milieu. The AS of BiVits® ACTIVA Recovery was much greater than the sum of AS of the individual components, indicating a synergistic effect of the components.